Reformat and Upload a Gene List
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1.
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Open the original TruSeq Custom Amplicon design gene list file (*.csv) in a spreadsheet editor, such as Excel. |
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2.
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Delete the following columns: |
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3.
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Rename the TargetType column into Type and move it to the left of the Gene column. |
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4.
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Enter Chromosome in the header of the column to the right of the Gene column. |
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5.
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Enter Start in the header of the column to the right of the Chromosome column. |
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6.
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Enter End in the header of the column to the right of the Start column. |
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7.
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Rename the Gene column into Name. |
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8.
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Enter GENE_CDS for all CDS entries and GENE_EXON for all Exon entries in the Type column. |
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9.
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Enter GENE_CDS or GENE_EXON for each FullRegion entry in the Type column. Alternatively, use a coordinate input file to upload FullRegion entries. See Reformat and Upload a Coordinates File. |
NOTE
The full target region will not be covered when using GENE_CDS or GENE_EXON for FullRegion entries.
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10.
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The Chromosome, Start, and End columns can be empty. |
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11.
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Save and close the gene list file. |
Make sure to select the species that matches the original TruSeq Custom Amplicon design. For human TruSeq Custom Amplicon designs, select Human (hg19).
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13.
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Configure the new AmpliSeq for Illumina design by selecting the Sample Type and Max Amplicon Length that most closely match those of the original TruSeq Custom Amplicon design. |
Use the following table for selecting equivalent amplicon sizes between the two assay types:
150
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140
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175
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175
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250
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275
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425
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375
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14.
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Use the default setting for Stringency. |