bcl2fastq2 Conversion Software v2.20

This download contains the software, release notes, and software guide for the bcl2fastq2 Conversion Software v2.20.0, which can be used to both demultiplex data and convert BCL files to FASTQ file formats for downstream analysis. This software requires a Linux operating system.

The bcl2fastq2 Conversion Software v2.20.0 can be used to convert BCL files from MiniSeq, MiSeq, NextSeq, HiSeq, iSeq and NovaSeq sequening systems. For conversion of data generated on Illumina sequencing systems using versions of RTA earlier than RTA 1.18.54, use bcl2fastq v1.8.4.

The software is available as an RPM (*.rpm) for installation on CentOS/RedHat Linux distributions. A tarball (*.tar.gz) of the source code is also available for compilation on other Linux distributions. Installation instructions are provided in the Software Guide.