Requirements & Compatibility

Kit Requirements

Sequencing Primers for Dual-Indexed Libraries

Sequencing primers provided in the TruSeq Dual Index Sequencing Primer Box are required for sequencing the following libraries on the HiScanSQ:

  • Any Nextera libraries with any number of indices for single-read or paired-end runs. Use the single-read kit with single-read flow cells, and the paired-end kit with paired-end flow cells.
  • TruSeq HT libraries when performing a dual-indexed run on a single-read flow cell.

For instructions, see the HiScanSQ System User Guide.

Kits and Software

Cluster Kit Version

SBS Kit Version

Software Version

TruSeq Cluster Kit v3

TruSeq SBS Kit v3 

HCS v1.4 or later/RTA v1.12, or later

Kit

Software Version

TruSeq Dual Index Sequencing Primer Box

HCS v1.5 / RTA v1.13, or later

Supported BeadChip Kits

The HiScanSQ is compatible with the following types of BeadChips:

  • Infinium
  • Direct Hybridization
  • Universal BeadChips