HiSeq 2500 Rapid Run Kits

Catalog IDs: PE-402-4002, GD-402-4002, FC-402-4022, FC-402-4021, FC-402-4023, CT-403-2001

Kit Requirements

Performing a rapid run requires a single-use HiSeq Rapid Cluster Kit v2 and a single-use HiSeq Rapid SBS Kit v2. The kits contain a flow cell and reagents for clustering and sequencing on the HiSeq 2500 System in Rapid Run mode.

  • The cluster kit is available in available in paired-end (PE) and single-read (SR) versions.
  • The SBS kit is available in three sizes: 500-, 200-, and 50-cycles.

Alternatively, you can use the cBot 2 or cBot System to load two libraries onto the flow cell, one in each lane. This option requires the HiSeq Rapid Duo cBot Sample Loading Kit in addition to the HiSeq Rapid Cluster Kit v2 and HiSeq Rapid SBS Kit v2. The HiSeq Rapid Duo cBot Sample Loading Kit performs template hybridization and first extension on the cBot 2 or cBot. Clustering is then completed on the HiSeq 2500.

For other system requirements, dual indexing considerations, and compatible products, see HiSeq 2500 Requirements & Compatibility.

Contents and Storage

When you receive your kits, promptly store kit components at the indicated temperatures to ensure proper performance:

HiSeq Rapid Cluster Kit v2

HiSeq Rapid Cluster Kit v2 reagents are shipped in one box on dry ice. A two-lane rapid flow cell and gaskets are also included.

PE Cluster Kit, Store at -25°C to -15°C

Quantity

Reagent

Description

1

AMS

Fast Amplification Mix

1

FDR

Fast Denaturation Reagent

1

FLM1

Fast Linearization Mix 1

1

FLM2

Fast Linearization Mix 2

1

FPM

Fast Premix

1

FRM

Fast Resynthesis Mix

1

HP10

Read 1 Primer

1

HP11

Read 2 Primer

1

HP12

i7 Index Primer

1

HT1

Hybridization Buffer

SR Cluster Kit, Store at -25°C to -15°C

Quantity

Reagent

Description

1

AMS

Fast Amplification Mix

1

FDR

Fast Denaturation Reagent

1

FLS

Fast Linearization Solution

1

FPM

Fast Premix

1

HP9

i5 Index Primer

1

HP10

Read 1 Primer

1

HP12

i7 Index Primer

1

HT1

Hybridization Buffer

HiSeq Rapid SBS Kit v2

HiSeq Rapid SBS Kit v2 reagents are shipped in two boxes: one is shipped at room temperature and the other is shipped on dry ice. Reagents are sensitive to light. Store the reagent cartridge in a dark location away from light.

Reagents are labeled with a three-letter acronym plus a numeric designator that indicates the kit size. For example, if you are using CRM-50, the CRM was provided in a 50-cycle kit.

SBS Kit Box 1

Quantity

Reagent

Description

Storage Temperature

1

CWM

Cleavage Wash Mix

15°C to 30°C

1

PW1

Wash Buffer

-25°C to 30°C

2

USB

Universal Sequencing Buffer

15°C to 30°C

SBS Kit Box 2, Store at -25°C to -15°C

Quantity

Reagent

Description

1

IMT

Incorporation Master Mix

1

CRM

Cleavage Reagent Mix

1

USM

Universal Scan Mix

HiSeq Rapid Duo cBot Sample Loading Kit

Reagents provided in the HiSeq Rapid Duo cBot Sample Loading Kit are shipped in one box on dry ice.

Sample Plate Box, Store at -25°C to -15°C

Quantity

Reagent

Description

1

cBot Rapid Duo Sample Plate

cBot reagent plate