Catalog IDs: PE-102-1001, PE-102-1002
The Paired-End Sample Preparation protocol prepares libraries of DNA fragments for paired-end sequencing. Adapter sequences are added onto the ends of DNA fragments. The two adapters contain sequences that are complimentary to the two surface-bound amplification primers on the flow cells.
Libraries prepared with the Paired-End DNA Sample Kit can be sequenced on the HiSeq 2000, HiSeq 1000, HiScanSQ, or Genome Analyzer.
This protocol is optimized for 1–5 µg of purified DNA (5 µg recommended) The DNA should be as intact as possible, with an OD260/280 ratio of 1.8–2.0.