Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria)

Catalog IDs: MRZGP126

The Ribo-Zero rNA Removal Kit (Gram-Positive Bacteria) will be discontinued on November 2nd, 2018. Alternatives are available from other suppliers. Illumina remains committed to providing customers with high-quality support and service.

Product Description

The Ribo-Zero (Gram-Positive Bacteria) kit is compatible with gram-positive bacteria samples. This kit removes bacterial rRNA.

Performance Specifications

Compare rRNA content before and after a Ribo-Zero reaction to assess the depletion of rRNA in total RNA.

A Ribo-Zero reaction removes:

  • > 99% of large cytoplasmic rRNA and large mitochondrial and chloroplast rRNA, as applicable.
  • > 85% to 95% of small rRNA (eg, 5S).

The success of rRNA removal depends on factors such as species, sample quality, and sequence homology between the rRNA sample and removal solution, especially with 5S rRNA removal. Use the Epicentre RNAMatchMaker at epibio.com/rnamatchmaker to verify sample and kit sequence homology sequence homology.

Input Requirements

This protocol supports rRNA depletion from 1–5 µg total RNA samples. Use a fluorometric-based method to quantify input.