Catalog IDs: MRZPL116, MRZPL1224, MRZSR116
The Ribo-Zero (Plant Leaf) kit is compatible with plant leaf tissue samples and the Ribo-Zero (Plant Seed/Root) kit is compatible with plant seed or root tissue. These kits remove eukaryotic cytoplasmic rRNA, mitochondrial rRNA, and chloroplast rRNA.
Compare rRNA content before and after a Ribo-Zero reaction to assess the depletion of rRNA in total RNA.
A Ribo-Zero reaction removes:
The success of rRNA removal depends on factors such as species, sample quality, and sequence homology between the rRNA sample and removal solution, especially with 5S rRNA removal. Use the Epicentre RNAMatchMaker at epibio.com/rnamatchmaker to verify sample and kit sequence homology sequence homology.
This protocol supports rRNA depletion from input of 500 ng–2.5 µg total RNA. Use a fluorometric-based method to quantify input.