Ribo-Zero rRNA Removal Kit (Plant)

Catalog IDs: MRZPL116, MRZPL1224, MRZSR116

The Ribo-Zero rRNA Removal Kit (Plant) will be discontinued on November 2nd, 2018. The TruSeq Stranded Total RNA Library Prep Plant is the recommended replacement product. Illumina remains committed to providing customers with high-quality support and service.

Product Description

The Ribo-Zero (Plant Leaf) kit is compatible with plant leaf tissue samples and the Ribo-Zero (Plant Seed/Root) kit is compatible with plant seed or root tissue. These kits remove eukaryotic cytoplasmic rRNA, mitochondrial rRNA, and chloroplast rRNA.

Performance Specifications

Compare rRNA content before and after a Ribo-Zero reaction to assess the depletion of rRNA in total RNA.

A Ribo-Zero reaction removes:

  • > 99% of large cytoplasmic rRNA and large mitochondrial and chloroplast rRNA, as applicable.
  • > 85% to 95% of small rRNA (eg, 5S).

The success of rRNA removal depends on factors such as species, sample quality, and sequence homology between the rRNA sample and removal solution, especially with 5S rRNA removal. Use the Epicentre RNAMatchMaker at epibio.com/rnamatchmaker to verify sample and kit sequence homology sequence homology.

Input Requirements

This protocol supports rRNA depletion from input of 500 ng–2.5 µg total RNA. Use a fluorometric-based method to quantify input.