TruSeq Amplicon - Cancer Panel FAQs

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  • Input


  • Yes, the TruSeq Amplicon - Cancer Panel has been optimized for use with DNA derived from FFPE sources. A simple qPCR-based quality control test (WG-321-1001, Illumina FFPE QC Kit) is recommended to ensure maximum sample success.

    Yes, the TruSeq Amplicon - Cancer Panel has been optimized for use with DNA derived from FFPE sources. The input amount depends on the ∆Cq. For more information, see the TruSeq FFPE DNA Library Prep QC Reference Guide (part # 15067391).

  • Protocol


  • You can use the Illumina FFPE QC Assay (WG-321-1001) to determine whether your samples meet the minimum quality criteria for inclusion in a Cancer Panel experiment. This protocol is qPCR based, where a Ct (threshold cycle) cut-off allows you to determine whether your samples will succeed in the TruSeq Amplicon assay or not. This measurement represents how well DNA amplifies and can be used to infer DNA quality. Testing has demonstrated that over 80% of samples passing the Illumina FFPE QC Assay protocol are expected to succeed in the TruSeq Amplicon assay.

    Use the Qiagen QIAamp DNA FFPE Tissue Kit (catalog # 56404).