Archived Webinars

Workflow Topic Date
Sample Preparation AmpliSeq for Illumina: Introduction

We will introduce our new line of amplicon products: AmpliSeq for Illumina- compatible with Illumina next-generation sequencing platforms. This webinar is targeted at users interested in moving to this amplicon chemistry. We will discuss the following topics:
- Available Fixed Panels
- Library Preparation Workflow
- Introducing the new Design Studio
- Overview of analysis options
Bring your questions to the discussion or send them in advance to techsupport@illumina.com.

Note: You will be given an option to call in, receive a call back, or stream webinar audio with high speed internet over the computer. Audio quality is best over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Sample Preparation Assessing the Success of your Amplicon Sequencing Project

Are you planning on running an Illumina amplicon library prep kit and wondering how to evaluate your data? This webinar is targeted to those who are new to amplicon sequencing and would like to learn more about the data output and assessment. We will cover the following subjects:
-Overview of an Illumina amplicon workflow
-Expected kit specifications
-Illumina options for amplicon analysis
-Output metrics and reports generated by the Illumina analysis platforms.

Note: You will be given an option to receive a call back, call in, or stream webinar audio with high speed internet over the computer. Please keep in mind that audio quality is highest over the telephone. You will need speakers or a headset to hear streaming audio.

 

More Info +
View
Analysis Software BaseSpace Sequence Hub: New Features

This webinar is targeted at users who have previously used the BaseSpace Sequence Hub, and will go over the following topics:
- Changes to the BaseSpace Sequence Hub web interface
- Working with My Data
- Understanding BioSamples

Note: You will be given an option to receive a call back, call in, or stream webinar audio with high speed internet over the computer. Please keep in mind that audio quality is highest over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Analysis Software Bcl2Fastq v2+: Introduction

Bcl2Fastq is a Linux-based software that converts base call files generated from an Illumina sequencing run to FASTQ files as well as demultiplex samples. This webinar focuses on Bcl2fastq v2+ and is targeted for new to intermediate users who are interested in the configurable options that this command line program allows. We will cover the following topics:
- Requirements to run Bcl2Fastq
- Command line basics
- Commonly used options
- Frequently asked ‘How To….’ questions.

Bring your questions to the discussion or send them in advance to techsupport@illumina.com.

Note: You will be given an option to call in, receive a call back, or stream webinar audio with high speed internet over the computer. Audio quality is best over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Analysis Software DesignStudio: Introduction

Have a targeted sequencing project in mind? This webinar is targeted toward new and intermediate users and will focus on using Illumina’s DesignStudio tool to design a custom targeted sequencing assay. We will cover the following subjects:
- How to use DesignStudio
- Workflow and how to create a design for: AmpliSeq for Illumina, Nextera Rapid Capture, TruSeq Targeted RNA Expression
- Tips and Tricks for a successful design

Note: You will be given an option to receive a call back, call in, or stream webinar audio with high speed internet over the computer. Please keep in mind that audio quality is highest over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Sample Preparation Enrichment Sequencing Part I: Tips for Successful Library Prep with Nextera Rapid Capture and TruSight Enrichment

The first of a 3-part webinar series will cover Illumina enrichment, with a focus on Nextera Rapid Capture and TruSight Enrichment. Join our Technical Application Scientists in a discussion of how Nextera Rapid Capture and TruSight enrichment kits work, and learn important considerations starting at the planning of your experiment through generation of your final library. This webinar is targeted at those who are new to sequencing and plan on running any of our new enrichment kits. You can send any specific questions you have ahead of time to TSWEBEX@illumina.com.

Note: You will be given an option to receive a call back, call in, or stream webinar audio with high speed internet over the computer. Please keep in mind that audio quality is highest over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Sample Preparation Enrichment Sequencing Part II: Tips for a Successful Library Prep with TruSeq Exome and Rapid Exome

The second part of a 3-part webinar series on Illumina enrichment will cover the two newest kits for Illumina enrichment, TruSeq Exome and TruSeq Rapid Exome. Join our Technical Application Scientists in a discussion of how Illumina enrichment kits work, and learn important considerations starting at the planning of your experiment through generation of your final library. This webinar is targeted at those who are new to sequencing and plan on running any of our new enrichment kits. You can send any specific questions you have ahead of time to TSWEBEX@illumina.com.
Note: You will be given an option to receive a call back, call in, or stream webinar audio with high speed internet over the computer. Please keep in mind that audio quality is highest over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Analysis Enrichment Sequencing Part III: Sequencing and Data Analysis

The third part of a 3-part webinar series on Illumina enrichment is targeted to those who are new to sequencing and plan on running any of Illumina's new enrichment kits. We discuss how to run enrichment samples on Illumina sequencing platforms and the downstream data analysis options, as well as instrument platforms, analysis options and helpful troubleshooting tips.

 

More Info +
View
Instrumentation FASTQ Processing Tools for Data Analysis

FASTQ files store biological sequence and quality information, and are key for downstream data analysis pipelines. Several steps are necessary during FASTQ generation to ensure optimal data analysis. This webinar is targeted at new and intermediate users including biologists intending to understand output of basic bioinformatics tools used for processing FASTQs. We will cover the following topics:
- What is a FASTQ file
- Overview of Illumina FASTQ generation tools
- FASTQ processing such as adapter trimming, masking, quality trimming, read stitching, etc
- How to best use FASTQC and FASTQ toolkit BaseSpace Lab apps on BaseSpace Sequence Hub*
- Highlight the differences between Illumina run performance metrics and output generated by third party tools such as FASTQC.

Note: You will be given an option to call in, receive a call back, or stream webinar audio with high speed internet over the computer. Audio quality is best over the telephone. You will need speakers or a headset to hear streaming audio.

*BaseSpace Labs Apps are developed using an accelerated development process in order to make them available to BaseSpace users faster than conventional Illumina Apps. Illumina may provide support for BaseSpace labs Apps at its sole discretion. BaseSpace Labs Apps are provided AS-IS without any warranty of any kind. BaseSpace Labs Apps are used at the user’s sole risk. Illumina is not responsible for any loss of data, incorrect results, or any costs, liabilities, or damages that may result from the use of a BaseSpace Labs App.

More Info +
View
Sample Preparation Introduction to Amplicon and 16S Metagenomics Sequencing

We will provide an overview of amplicon sequencing, including library preparation methods and sequencing. This webinar is targeted to new users and covers the following topics:
-What defines an amplicon library
-Kits and protocols for amplicon libraries
-Amplicon sequencing runs and parameters

Note: You will be given an option to call in, receive a call back, or stream webinar audio with high speed internet over the computer. Audio quality is best over the telephone. You will need speakers or a headset to hear streaming audio.

 

More Info +
View
Analysis Software Introduction to BaseSpace Sequence Hub

Illumina Technical Support invites you to learn some basics about the functionality and navigation of BaseSpace, Illumina’s cloud based sequencing data analysis solution. We will address topics such as the difference between runs and projects, how to work with data, and how to share your data using this tool. Instrument setup, development of new apps, and API programming will not be discussed in this webinar.
Note: You will be given an option to call in, receive a call back, or stream webinar audio with high speed internet over the computer. Audio quality is best over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Analysis Software Introduction to BlueFuse Multi – Cytochip Module

This webinar will introduce customers to BlueFuse Multi. It will describe how to install and set-up the software for use with compatible Illumina products. There will also be an introductory overview of functionality of the software for the cytochip module. A question and answer session will immediately follow the presentation.

More Info +
View
Instrumentation Introduction to HiSeq 3000/4000

IlluminaTechnical Support invites users to join us for a discussion of the HiSeq 3000/4000 systems. This webinar is targeted toward new users with a basic understanding of the Illumina sequencing workflow and will review:
-End to End HiSeq 3000/4000 workflow
-Patterned flow cell technology
-ExAmp Clustering Tips and Tricks
-Tips and Best Practices 

Note: You will be given an option to call in, receive a call back, or stream webinar audio with high speed internet over the computer. Audio quality is best over the telephone. You will need speakers or a headset to hear streaming audio.

 

More Info +
View
Analysis Introduction to Key Concepts in Sequencing Data Analysis

Illumina Technical Support invites you to join us for an introductory presentation and discussion on the concepts and general approaches used in analysis of Illumina sequencing data.  This webinar is targeted to those new to Illumina Next Generation sequencing, and covers the basic analysis approaches used in de novo assembly, RNA sequencing, and SNP finding.  Basic concepts in experimental design are also discussed.

More Info +
View
Analysis Introduction to Metagenomics Data Analysis Concepts

This webinar features an introduction to 16S rRNA analysis, with a specific focus on the MiSeq analysis workflow.

More Info +
View
Instrumentation Introduction to MiniSeq

Please join us for a technical presentation of Illumina’s newest benchtop sequencer, the MiniSeq. This webinar will educate new and experienced Next-Generation Sequencing users to how this system works, its new features, how dual indexing is performed, best practices and introductory guidance on run analysis.

Note: You will be given an option to receive a call back, call in, or stream webinar audio with high speed internet over the computer. Please keep in mind that audio quality is highest over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Instrumentation Introduction to NextSeq 500

In this webinar we cover topics related to running the NextSeq 500 for new users. We will discuss how the system works, how dual indexing is performed, and provide introductory guidance on run analysis.

More Info +
View
Sample Preparation Introduction to Sequencing FFPE Samples

FFPE is a widely used process to conserve tissues and generally contain partially degraded or limited genomic material. Specific considerations are required to sequence these challenging samples. This webinar is targeted towards both new and experienced users, with a basic knowledge of working with FFPE samples and will go over the following topics:

- A basic introduction to FFPE sample types

- The nucleic acid extraction process

- Library Preparation options  

Note: You will be given an option to call in, receive a call back, or stream webinar audio with high speed internet over the computer. Audio quality is best over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Sample Preparation Introduction to Single-Cell RNA Sequencing

We will introduce why researchers are using single-cell RNA sequencing (RNA-Seq) and our solution, the Illumina™ | Bio-Rad™ ddSEQ™ Single-Cell Isolator and SureCell™ WTA 3' Library Prep Kit. This webinar will discuss the library prep workflow, including important considerations for planning your experiment through generation of your final library. We will also discuss sequencing and analyzing your experiment. This webinar is targeted at new users with a basic knowledge of next generation sequencing and will go over the following topics:
- Single-Cell RNA-Seq
- Sample preparation workflow
- Tips for a successful library prep
- Sequencing considerations
-Analysis workflow options

Bring your questions to the discussion or send them in advance to techsupport@illumina.com.

Note: You will be given an option to call in, receive a call back, or stream webinar audio with high speed internet over the computer. Audio quality is best over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Sample Preparation Introduction to TruSeq Bovine Parentage Panel Library Prep and Sequence Genotyper Analysis Tool

We will learn how libraries are generated by our TruSeq Bovine Parentage Sequencing Panel and discuss important considerations starting at the planning of your experiment through generation of your final library. We will also discuss how to use the new analysis tool for this kit; Sequence Genotyper. This webinar is targeted at new users with a basic knowledge of next generation sequencing and will go over the following topics:
-Sample Preparation workflow
-Tips for a successful library prep
-Importing files for analysis in Sequence Genotyper

Note: You will be given an option to call in, receive a call back, or stream webinar audio with high speed internet over the computer. Audio quality is best over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Sample Preparation Introduction to TruSeq Synthetic Long-Reads Part I: Library Preparation

Part 1 of a 2 part webinar series- Introduction to TruSeq Synthetic Long-Reads.

Would you like to learn about how Illumina’s newest long-read sequencing solution can enable your research?  In this webinar, we discuss the TruSeq Synthetic Long Reads Library Preparation approach, the ancillary equipment and reagents that are required, and how to prepare for your first run.

Part 2 of the series; Sequencing and Data Analysis, will be covered in the subsequent webinar.

More Info +
View
Sample Preparation Introduction to TruSeq Synthetic Long-Reads Part II: Sequencing and Data Analysis

Part II of a 2-part webinar series- Introduction to TruSeq Synthetic Long-Reads.

Part I: Library Preparation, is a pre-requisite for this webinar.

In this webinar, we discuss best practices for post-library preparation sequencing and analysis.  Learn how to use and what to expect from the TruSeq Synthetic Long-Read Assembly App offered in BaseSpace.  Not a bioinformatics seminar. 

More Info +
View
Analysis Software Introduction to VariantStudio

Illumina Technical Support invites you to join us for an introduction to the VariantStudio software. We will review how to import data into VariantStudio, perform annotations and create reports. A basic familiarity with VariantStudio would be an advantage and we welcome questions following the presentation.

Note: You will be given an option to receive a call back, call in, or stream webinar audio with high speed internet over the computer. Please keep in mind that audio quality is highest over the telephone. You will need speakers or a headset to hear streaming audio.

 

 

More Info +
View
Analysis Software Local Run Manager 2.0 (Research Use Only): Introduction - MiSeq and Off-Instrument

Local Run Manager is a Windows-based software for Illumina benchtop sequencing data that includes local analysis options as well as run and user management. This webinar targets new and intermediate users of the software. It will provide an overview of the Research Use Only (RUO) version of Local Run Manager 2.0, including the off-instrument version and the version bundled with MiSeq Control Software 3.0. This webinar does not cover the Dx versions of the software.

We will cover the following topics:
- Features of Local Run Manager 2.0
- Available versions and analysis options
- How to set up runs and analyze data
- Interaction with on-instrument control software
- Comparison to other Illumina analysis software

Bring your questions to the discussion or send them in advance to techsupport@illumina.com. 

Note: You will be given an option to call in, receive a call back, or stream webinar audio with high speed internet over the computer. Audio quality is best over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Analysis Software Local Run Manager 2.0 (Research Use Only): Introduction - MiSeq, iSeq 100, and Off-Instrument

Local Run Manager is a Windows-based software for Illumina benchtop sequencing data that includes local analysis options as well as run and user management. This webinar targets new and intermediate users of the software. It will provide an overview of the Research Use Only (RUO) version of Local Run Manager 2.0, including the off-instrument version and the version bundled with MiSeq Control Software 3.0, and iSeq Control Software. This webinar does not cover the Dx versions of the software.

We will cover the following topics:
- Features of Local Run Manager 2.0
- Available versions and analysis options
- How to set up runs and analyze data
- Interaction with on-instrument control software
- Comparison to other Illumina analysis software

Bring your questions to the discussion or send them in advance to techsupport@illumina.com. 

Note: You will be given an option to call in, receive a call back, or stream webinar audio with high speed internet over the computer. Audio quality is best over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Sample Preparation Nextera DNA Flex Library Prep Kit: Introduction

Illumina Technical Support invites you to join us for an introductory presentation on the new Nextera DNA Flex library preparation kit. This webinar is geared towards new and intermediate users with a basic knowledge of next generation sequencing. We will cover the following topics:
- Sample to library workflow
- Tips for a successful library prep

Bring your questions to the discussion or send them in advance to techsupport@illumina.com

Note: You will be given an option to call in, receive a call back, or stream webinar audio with high speed internet over the computer. Audio quality is best over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Sample Preparation Nextera XT: Sample Preparation and Usage

Illumina Technical Support invites you to join us for a presentation and discussion on the Nextera XT sample preparation process. This webinar is targeted to those customers who are planning to work with the Nextera XT Sample Preparation kit. We will review the protocol, providing information on best practices for critical steps. This will be followed by a comparison of the Nextera XT kit to the Nextera DNA kit, focusing on the applications recommended with each kit. A question and answer session will immediately follow the presentation.

Note: You will be given an option to call in, receive a call back, or stream webinar audio with high speed internet over the computer. Audio quality is best over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Instrumentation NovaSeq: Introduction

Illumina Technical Support invites you to join us for a discussion of the new NovaSeq platform. This webinar is targeted towards users with a basic understanding of the Illumina sequencing workflow. This webinar will cover:
-Features of NovaSeq
-End to End workflow
-Library preparation considerations
-Guidance on Run analysis
-Tips and Best Practices

More Info +
View
Analysis Software Prep Tab on BaseSpace: The Illumina Cloud-Based Solution for Managing Samples

During this presentation you will learn the basic functionalities of Prep Tab such has creating and importing biological samples, creating custom library prep kits, importing libraries, highlight differences between runs and projects. Instrument setup, development of new apps, and programming will not be discussed in this webinar. A question and answer will immediately follow the presentation.


More Info +
View
Experimental Design RNA-Seq Part I: Introduction

Part 1 of a 4-Part RNA-Seq Webinar Series

In this webinar we will describe key considerations when planning RNA sequencing experiments. We will cover RNA sample preparation through sequencing to basic data analysis. We assume a basic understanding of Illumina sequencing technology, but no prior knowledge of RNA sequencing is required. The webinar is targeted towards both new and experienced users, and will include a question and answer session.

 

More Info +
View
Sample Preparation RNA-Seq Part II: Kits

Part 2 of a 4-part RNA-Seq webinar series.

Following RNA-Seq Part I, we will review sample preparation kit options for total RNA, mRNA, and targeted RNA sequencing projects. Great for both new and experienced users, we will assume a basic understanding of Illumina sequencing technology, although no knowledge of sample prep is required. In particular, we’ll discuss:
-How to identify which kit is right for your experiment
-What are the expected input and outputs
-Where to go for additional resources

Bring your questions or send them in advance to techsupport@illumina.com.

Note: You will be given an option to call in, receive a call back, or stream webinar audio with high speed internet over the computer. Audio quality is best over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Sample Preparation RNA-Seq Part III: Best Practices

Part 3 of a 4-part RNA-Seq webinar series.

In the third part of our RNA-Seq Webinar series, we dive into best practices for Illumina’s TruSeq RNA kits. This webinar is aimed at both new and experienced users with a basic understanding of Illumina Sequencing technology and workflow. We will focus on TruSeq RNA v2, TruSeq Stranded mRNA, and TruSeq Stranded Total RNA and discuss:
- Bead handling
- Quantification methods
- Library QC
- Troubleshooting

Bring your questions or send them in advance to techsupport@illumina.com.

Note: You will be given an option to call in, receive a call back, or stream webinar audio with high speed internet over the computer. Audio quality is best over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Analysis Software RNA-Seq Part IV: Introduction to Analysis

In this webinar, we will discuss the RNA-Seq analysis options available on BaseSpace including the RNA-Seq Alignment/Cufflinks, TopHat/Cufflinks, and RNA Express apps. We assume both a basic understanding of Illumina sequencing technology and knowledge of the general library prep workflow for the TruSeq RNA kits. The webinar is targeted towards both new and experienced users, and will include a question and answer session. Please note that small RNA sequencing analysis will not be covered in this webinar. We encourage you to attend Part I: Introduction, Part II: Kits and Part III: Best Practices, prior to this webinar.

Note: You will be given an option to receive a call back, call in, or stream webinar audio with high speed internet over the computer. Please keep in mind that audio quality is highest over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Analysis Software Sequence Analysis Viewer (SAV) 101: What Does it All Mean?

The Sequencing Analysis Viewer (SAV) Software is an application to view important quality metrics generated during sequencing runs. This webinar will provide a guided tour for beginners on how to use SAV, as well as tips and tricks for reviewing the most useful information for sequencing runs. This webinar is targeted at new users and will go over the following topics:
-How to load data into SAV
-What metrics are available in each tab of the software
-Understanding which are the most valuable metrics for run review and where to find them

Note: You will be given an option to call in,
receive a call back, or stream webinar audio with high speed internet over the computer. Audio quality is best over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Instrumentation Sequencing Considerations for Low Diversity Libraries

Low diversity libraries, such as those used for amplicon sequencing, can present challenges on Illumina sequencing systems and may skew software performance and data accuracy resulting in poor quality runs. This webinar is ideal for new and intermediate users interested in considerations for the sequencing of low diversity libraries and will cover the following topics:
-Understanding and visualizing base diversity in Sequencing Analysis Viewer (SAV)
-Strategies to optimize low diversity sequencing data
-% PhiX spike-in and cluster density recommendations for different sequencing platforms
-Overview of base calling and quality score calculations

Bring your questions to the discussion or send them in advance to techsupport@illumina.com.

Note: You will be given an option to call in, receive a call back, or stream webinar audio with high speed internet over the computer. Audio quality is best over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Experimental Design Small RNA-Seq Part I: Introduction

Part 1 of a 2-part Small RNA-Seq Webinar series. In this webinar we describe key considerations when planning small RNA sequencing experiments. We cover RNA isolation, library preparation, sequencing and data analysis. We assume a basic understanding of Illumina sequencing technology, but no knowledge of library preparation is required. The webinar is targeted towards both new and experienced users, and will include a question and answer session. Please stay tuned for Part II: Best Practices, which will be presented shortly after this webinar.

Note: You will be given an option to receive a call back, call in, or stream webinar audio with high speed internet over the computer. Please keep in mind that audio quality is highest over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Sample Preparation Small RNA-Seq Part II: Best Practices

Part 2 of the the 2-part Small RNA-Seq webinar series.

In this webinar we discuss important best practices for generating successful small RNA libraries with the TruSeq Small RNA library preparation kit. We also provide advice on assessing input material and evaluating final libraries. We assume a basic understanding of Illumina sequencing technology, but no knowledge of library preparation is required. The webinar is targeted towards both new and experienced users, and will include a question and answer session. We encourage you to attend Small RNA Sequencing Part I: Introduction
before this webinar.

Note: You will be given an option to receive a call back, call in, or stream webinar audio with high speed internet over the computer. Please keep in mind that audio quality is highest over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Instrumentation iSeq 100: Introduction

Illumina Technical Support invites you to join us for a discussion of the new iSeq 100 systems. This webinar is targeted to new users with a basic understanding of the Illumina sequencing workflow. This webinar will cover:
- Features of iSeq 100
- End to end workflow
- Review the new Illumina technologies that power the iSeq 100
- Review the troubleshooting path when working with Technical Support

Bring your questions to the discussion or send them in advance to techsupport@illumina.com.

Note: You will be given an option to receive a call back, call in, or stream webinar audio with high speed internet over the computer. Please keep in mind that audio quality is highest over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View
Analysis TruSight Tumor 170 Part I: DNA Analysis Workflow Overview

The TruSight Tumor 170 BaseSpace Sequence Hub app is a comprehensive analysis solution for sequencing data generated from DNA and RNA libraries prepared with the TruSight Tumor 170 library prep kit. This webinar is geared toward new and intermediate users of the TruSight Tumor 170 BaseSpace app who want a better understanding of the analysis workflows and output files.
The webinar will cover the following topics:
- The TruSight Tumor 170 DNA analysis workflow
- The format and content of the TruSight Tumor 170 BaseSpace app’s output files

Bring your questions to the discussion or send them in advance to techsupport@illumina.com

Note: You will be given an option to call in, receive a call back, or stream webinar audio with high speed internet over the computer. Audio quality is best over the telephone. You will need speakers or a headset to hear streaming audio.

More Info +
View