Recommandation des densités de cluster selon les types de plateforme de séquençage Illumina utilisant des flow cell non structurées

La densité de cluster est un facteur important pour optimiser la qualité et le rendement des données. Le tableau suivant répertorie les densités de cluster brutes recommandées pour des librairies équilibrées (telles que PhiX):

Plateforme	Mode/Réactifs	Densités de Cluster brutes optimales
HiSeq™	High Output, v3	750-850 K/mm²
	High Output, v4	950-1050 K/mm²
	Rapid Run, v2	850-1000 K/mm²
MiSeq™	v2	1000-1200 K/mm²
MiSeq™	v3	1200-1400 K/mm²
MiniSeq™	Mid and High Output	170-220 K/mm²
NextSeq 500/550™	Mid and High Output	170-220 K/mm²

*High: Débit élevé; Mid: Débit moyen

Les systèmes iSeq ™ 100, NextSeq ™ 1000 / NextSeq ™ 2000, HiSeq ™ 3000 / HiSeq ™ 4000, HiSeq ™ X et NovaSeq ™ 6000 utilisent des flow cell structurées ce qui se traduit par une densité de cluster fixe, même si tous les nano-puits ne sont pas occupés. Dans ses systèmes, la surveillance du pourcentage de passage du filtre des clusters (% cluster passing filter) est une meilleure mesure de l’occupation potentielle du cluster.

Veuillez trouver ci-dessous d’autres liens pour plus d’information:

Plotting % Occupied by % Pass Filter to optimize loading concentration for NovaSeq 6000 and iSeq 100 platforms

Best practices to improve clusters passing filter and yield when using patterned flow cells

Calculating Percent Passing filter for Patterned and Nonpatterned Flow Cells

Recommandation des densités de cluster selon les types de plateforme de séquençage Illumina utilisant des flow cell non structurées

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Recommandation des densités de cluster selon les types de plateforme de séquençage Illumina utilisant des flow cell non structurées

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