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Create an AmpliSeq for Illumina Design from an Existing TruSeq Custom Amplicon Design/Use Target Regions from a TruSeq Custom Amplicon Design/Reformat and Import Target Regions Into an AmpliSeq for Illumina Design

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Reformat and Import Target Regions Into an AmpliSeq for Illumina Design

1. Open the TruSeq Custom Amplicon design target regions file (*.bed) in a spreadsheet editor, such as Excel.
2. Delete the fifth and sixth columns from the file.
3. Make sure that all target names are 20 alphanumeric characters or less. Hyphens, periods, underscores, and whitespaces are allowed.

The name field can be empty.

4. Save and close the target regions file.
(missing or bad snippet)
5. Upload the TruSeq Custom Amplicon design target regions file into the AmpliSeq for Illumina design. See Add Targets by Uploading Files.