This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets.
After completing this course you will be able to describe the TruSeq Targeted RNA Expression Kit, the steps to order a custom oligo pool, the steps in the TruSeq Targeted RNA Expression protocol, best practices, and how to analyze and normalize data.
After completing this course you will understand best practices for all TruSeq Sample Preparation Kits including liquid handling, AMPure XP handling, avoiding cross-contamination, and temperature considerations. You will also learn about specific information about DNA and RNA best practices.
Recorded Webinar (March 2020) | Have a targeted sequencing project in mind? This webinar is targeted toward new and intermediate users looking to design a custom targeted sequencing assay in DesignStudio. We wiill show you how to use the software, and share tips and tricks to create a design for AmpliSeq for Illumina, Nextera Rapid Capture, and TruSeq Targeted RNA Expression.
Recorded Webinar (October 2020) | Part 1 of a 3-Part RNA-Seq Webinar Series. This session will review sample preparation kit options for RNA input. An introduction to the new Illumina Stranded mRNA Ligation, Illumina Stranded Total RNA Ligation with Ribozero Plus, and Illumina RNA prep with Enrichment Tagmentation workflows will be provided along with a comparison to established Illumina kits. This webinar is targeted towards both new and experienced users and will cover the following topics: key considerations when planning RNA sequencing experiments, how to identify which kit is right for your experiment, what are the expected input and outputs, and where to find additional resources.
Recorded Webinar (November 2020) | In the second part of our RNA-Seq Webinar series, we dive into best practices for Illumina’s RNA kits. This webinar is aimed at both new and experienced users with a basic understanding of Illumina Sequencing technology and workflow. We will focus on TruSeq RNA v2, TruSeq Stranded mRNA, Illumina Stranded mRNA Ligation, TruSeq Stranded Total RNA, Illumina Stranded Total RNA Ligation and Illumina RNA prep with Enrichment Tagmentation. We will cover the following topics: bead handling, quantification methods, library QC and troubleshooting.
Upcoming Webinar | Does my library look good to sequence? What’s that peak? This webinar will address how to use the Agilent BioAnalyzer to check library quality prior to sequencing, and troubleshoot sample preparation. This webinar is geared toward new and intermediate users, and will cover how to identify features of an ideal library trace, recognize potential issues, and prevent potential issues.
Apr 27, 2021 PST