TruSeq RNA Sample Prep Kit v2 FAQs

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  • General


  • TruSeq DNA and RNA v1 kits are no longer available for order. Contact your local Account Manager for assistance in transitioning to the latest TruSeq RNA and DNA Sample Prep Kits for sequencing projects.

    The TruSeq Stranded RNA HT adapter plate is not compatible with TruSeq RNA Libary Prep Kits as the protocol and the reagents are different.

    TruSeq DNA and RNA Sample Prep kits - Set A

    • V1—Contains enough reagents and adapters to process 48 samples. Each kit contains 6 unique indexes, each index sufficient for 8 individual samples. These indexes were optimized for lower plexity multiplexing of DNA samples. Indexes: 2, 4, 5, 6, 7, 12.
    • V2/LT—Contains enough reagents and adapters to process 48 samples. Each kit contains 12 unique indexes, each index sufficient for 8 individual samples. Indexes: 2, 4, 5, 6, 7, 12, 20, 21, 22, 23, 25, 27.

    TruSeq DNA and RNA Sample Prep kits - Set B

    • V1—Contains enough reagents and adapters to process 48 samples. Each kit contains 6 unique indexes, each index sufficient for 8 individual samples. Supplemental kit for use with Optimized Kit. Indexes: 1, 3, 8, 9, 10, 11
    • V2/LT—Contains enough reagents and adapters to process 48 samples. Each kit contains 12 unique indexes, each index sufficient for 8 individual samples. Supplemental kit for use with Optimized Kit. Indexes: 1, 3, 8, 9, 10, 11, 13, 14, 15, 16, 18, 19

    While Illumina supplies additional indexed adapters in the TruSeq Sample Prep v2 kits, the fill volumes are kept at 8 samples to minimize disruption to the protocols.  The v2 kits are limited by other components to 48 samples per kit. This also offers more flexibility for how customers use the indexed adapters.

    For some library preparations, AMPure XP beads are user-supplied from Beckman-Coulter Genomics. See the appropriate TruSeq DNA or RNA library prep guide for more information.

    Oligo-only kits are not offered for TruSeq DNA and RNA Library Preparation.

  • Sequencing


  • For runs on HiSeq Systems, creating and loading a sample sheet at the start of the run is optional. However, using a sample sheet allows you to view data shown on the indexing tab in the Sequencing Analysis Viewer (SAV) during the run. If you do not load a sample sheet at the start of a run in HCS, you will not be able to view indexing data in SAV.

    If you are setting up a run in MiSeq Control Software or analyzing indexed samples using CASAVA v1.8.2, a sample sheet is required. 

    Illumina recommends that you create the sample sheet using the Illumina Experiment Manager (IEM) prior to performing library prep in order to confirm appropriate index combinations.