The TruSight Tumor 26 Kit contains the following components and is shipped on dry ice unless specified otherwise below. As soon as you receive your kit, store the kit components at the specified temperatures in designated pre-amplification and post-amplification areas.
TruSight Tumor 26 Kit
Box 1, Pre-Amplification
This set of reagents contains formamide, an aliphatic amide that is a probable reproductive toxin. Personal injury can occur through inhalation, ingestion, skin contact, and eye contact. Dispose of containers and any unused contents in accordance with the governmental safety standards for your region. For more information, see the MSDS for this kit, at http://www.illumina.com/msds.
| Quantity |
Reagent |
Description |
Storage |
|---|
| 1 |
ACD1 |
Amplicon Control DNA 1 |
-25° to -15°C |
| 1 |
ACP1 |
Amplicon Control Oligo Pool 1 |
-25° to -15°C |
| 1 |
OHS1 |
Oligo Hybridization for Sequencing Reagent 1 |
-25° to -15°C |
| 1 |
ELM3 |
Extension Ligation Mix 3 |
-25° to -15°C |
| 1 |
PMM2 |
PCR Master Mix 2 |
-25° to -15°C |
| 1 |
TDP1 |
TruSeq DNA Polymerase 1 |
-25° to -15°C |
| 1 |
SW1 |
Stringent Wash 1 |
2° to 8°C |
| 1 |
UB1 |
Universal Buffer 1 |
2° to 8°C |
Box 1, Pre-Amplification
| Quantity |
Reagent |
Description |
Storage |
|---|
| 1 |
HT1 |
Hybridization Buffer |
-25° to -15°C |
| 4 |
EBT |
Elution Buffer with Tris |
Room temperature |
Box 3, TruSight Tumor Oligo Set, Pre-Amplification, Store at -25° to -15°C
This box is shipped at room temperature. As soon as you receive your kit, remove LNB1 from box 2 and store at 2° to 8°C in the post-amplification area. The filter plate should remain in the pre-amplification area at room temperature.
| Quantity |
Reagent |
Description |
|---|
| 1 |
QCP |
Quality Control Primers |
| 1 |
FPA |
TruSight Tumor Oligo Pool A |
| 1 |
FFB |
TruSight Tumor Oligo Pool B |
| 1 |
QCT |
Quality Control Template |
TruSight Tumor 26 Index Kit
Box 1, Pre-Amplification, Store at -25° to -15°C
| Quantity |
Description |
|---|
| 8 |
i5 Index Primers, A501 to A508 |
| 12 |
i7 Index Primers, A701 to A712 |
Box 2, Pre-Amplification, Store at Room Temperature
| Quantity |
Description |
|---|
| 32 |
i5 Index Tube Caps, White |
| 48 |
i7 Index Tube Caps, Orange |