Nextera Rapid Capture Custom Enrichment Kits Compatible Products

Additional Primers

When clustering libraries on the cBot or cBot 2 Systems and sequencing on the HiSeq 1000 or HiSeq 2000 Systems or in High Output mode on the HiSeq 1500 or HiSeq 2500 Systems, new primers are required whether performing a non-indexed, single-indexed, or dual-indexed run.

Use the TruSeq Dual Index Sequencing Primer Box for Paired End runs (catalog # PE-121-1003), which contains the required primers for sequencing (HP10, HP11, HP12).

Cluster Density

Quantifying a Nextera Rapid Capture Enrichment post-enriched library using a fluorometric method results in the following cluster densities. Results can vary based on your method of quantification.

Instrument Mode/Version Expected Cluster Density (mm2)
MiSeq v2* 800,000–900,000
  v3 1,200,000–1,400,000
NextSeq High Output Flowcell 200,000-240,000
  Mid Output Flowcell 200,000-240,000
HiSeq High Output 750,000–950,000
  Rapid Run 750,000–950,000

* Used for Nextera Rapid Capture Custom Enrichment only