With the introduction of the Nextera XT Index Kit v2, catalog no. FC-131-1002 has been obsolesced (Nextera XT Index Kit - 96 indexes, 384 samples).
Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, or cDNA in less than 90 minutes, with a low DNA input requirement.A Nextera XT Index Kit, Nextera XT Index Kit v2, or IDT for Illumina Nextera DNA UD Indexes Kit is required to complete the protocol, regardless of the sample pooling level used for sequencing. See Product Compatibility for more information.
This protocol is optimized for 1 ng of total gDNA. Use a fluorometric-based method to quantify input.