This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets.
After completing this course you will understand best practices for all TruSeq Sample Preparation Kits including liquid handling, AMPure XP handling, avoiding cross-contamination, and temperature considerations. You will also learn about specific information about DNA and RNA best practices.
What are the impacts of sequencing amplicon and low diversity libraries on Illumina instruments? An Illumina Field Applications Scientist discusses considerations for designing and performing amplicon sequencing.
An Illumina Field Applications Scientist discusses best practices for sequencing amplicons on Illumina instruments with high-quality results. We use Sequencing Analysis Viewer (SAV) to compare key metrics of amplicon sequencing runs to a standard PhiX run.
Many next-generation sequencing applications use the Polymerase Chain Reaction, or PCR, for DNA amplification. This video presents best practices to minimize the potential for PCR contamination in your experiment.
Recorded Webinar (March 2017) | Are you planning on running an Illumina amplicon library prep kit and wondering how to evaluate your data? This webinar is targeted to those who are new to amplicon sequencing and would like to learn more about the data output and assessment. We will cover the following subjects: overview of an Illumina amplicon workflow, expected kit specifications, Illumina options for amplicon analysis, output metrics and reports generated by the Illumina analysis platforms.
Recorded Webinar (December 2018) | FFPE is a widely used process to conserve tissues and generally contain partially degraded or limited genomic material. Specific considerations are required to sequence these challenging samples. This webinar is targeted towards both new and experienced users, with a basic knowledge of working with FFPE samples and will go over the following topics: A basic introduction to FFPE sample types, the nucleic acid extraction process, and library Preparation options.
Upcoming Webinar | Does my library look good to sequence? What’s that peak? Join Illumina Technical Support for a webinar on how to use the Agilent BioAnalyzer to check library quality prior to sequencing, and troubleshoot sample preparation. This webinar is geared toward new and intermediate users, and will cover the following topics: How to identify features of an ideal library trace, how to recognize potential issues, and how to prevent potential issues.
Sep 12, 2019 PST