TruSight Oncology 500 ctDNA v2 Contents & Storage

TruSight Oncology 500 ctDNA v2 Kit (20105899)

Box 1- Library Prep (Pre-Amp), Store at -25°C to -15°C
Quantity Reagent Description
1 ATL4 A-Tailing Mix 4
1 EPM4 Enhanced PCR Mix
1 ERP6 End Repair Mix 6
1 LIGX DNA Ligase X
1 RSB
Resuspension Buffer
Box 2 - Library Prep (Pre-Amp), Store at Room Temperature
Quantity Reagent Description
1 IPB Illumina Purification Beads
Box 3 - Enrichment (Post-Amp), Store at -25°C to -15°C
Quantity Reagent Description
1 EE1 Enrichment Elution Buffer 1
1 EEW Enhanced Enrichment Wash
1 EPM4 Enhanced PCR Mix
1 HP3 2 N NaOH
1 LNA1 Library Normalization Additives
1 NHB2 Hyb Buffer 2 + IDT NXT Blockers
1 OPD2 Enrichment Probe Panel
2 PPC3 PCR Primer Cocktail 3
1 RSB Resuspension Buffer
Box 4 - Enrichment (Post-Amp), Refer to Storage Temperatures in Table
Quantity Reagent Description Storage
1 EHB2 Enrich Hyb Buffer 2 2°C to 8°C
1 IPB Illumina Purification Beads Room temperature
1 LNB1 Library Normalization Beads 2°C to 8°C
1 LNS1 Library Normalization Storage Buffer 1 2°C to 8°C
2 LNW1 Library Normalization Wash 1 2°C to 8°C
2 SMB3 Streptavidin Magnetic Beads 2°C to 8°C