Illumina DNA Prep with Enrichment Dx Compatible Products

Sample Information

Before preparing libraries, record information about your samples using an option available for your sequencing system.

• MiSeqDx Instrument 
• NextSeq 550Dx Instrument
• NovaSeq 6000Dx Instrument

Library Prep

Index Kit

Illumina DNA Prep with Enrichment Dx includes one of the following index kits:

• Illumina Unique Dual Index Dx Set A
• Illumina Unique Dual Index Dx Set B

Blood and FFPE Input

To start the protocol from blood or formalin-fixed paraffin embedded (FFPE) tissue, use any validated extraction method. 

Sequencing Guidelines

For sequencing, regardless of the enrichment probe panel you are using, Illumina recommends setting up a paired-end run with 151 cycles per read (2 × 151) and 10 cycles per Index Read. If you would like fewer overlapped reads or less raw coverage, you can sequence down to 2 × 126 or 2 x 101.