Use Illumina Experiment Manager to record information about your samples, such as sample ID and which index adapters are used for pooling.
Using the HiSeq Rapid Duo cBot Sample Loading Kit to load a different library into each lane of the rapid flow cell also requires clustering on the cBot 2 or cBot. Otherwise, Rapid Run mode on the HiSeq 1500 System performs both cluster generation and sequencing.
Sequencing primers provided in the TruSeq Dual Index Sequencing Primer Box are required for sequencing the following libraries on the HiSeq 2500:
For more information, see HiSeq 2500 Primers.
|HiSeq Cluster Kit v4
|HiSeq SBS Kit v4
|HCS v2.2/RTA v1.18, or later
|TruSeq Cluster Kit v3
|TruSeq SBS Kit v3
|HCS v1.4/RTA v1.12, or later
|HiSeq Rapid PE Cluster Kit v2
HiSeq Rapid SR Cluster Kit v2
HiSeq Rapid Duo cBot Sample Loading Kit*
|HiSeq Rapid SBS Kit v2
|HCS v2.2.58/RTA v1.18, or later
* Also requires Version R recipes and cBot software v1.5, or later.
|cBot Software Version
|TruSeq Dual Index Sequencing Primer Box
|cBot Software v1.4.36, or later
cBot Recipe Installer v1.0.13
|HCS v1.5/RTA v1.13, or later