NextSeq 1000/2000 Sequencing System Documentation

System Documents

SIZE

DATE

NextSeq 1000/2000 Product Documentation

Instructions for operating and maintaining the NextSeq 1000/2000 Instrument.

View Online Help

NextSeq 1000/2000 Product Documentation

May 31, 2024

XLEAP-SBS Chemistry Quick Reference

Mar 11, 2024

NextSeq 1000/2000 Product Documentation Translated into Arabic

May 31, 2024

NextSeq 1000/2000 Product Documentation Translated into Brazilian Portuguese

May 31, 2024

NextSeq 1000/2000 Product Documentation Translated into Bulgarian

May 31, 2024

NextSeq 1000/2000 Product Documentation Translated into Danish

May 31, 2024

NextSeq 1000/2000 Product Documentation Translated into Dutch

May 31, 2024

NextSeq 1000/2000 Product Documentation Translated into Estonian

May 31, 2024

NextSeq 1000/2000 Product Documentation Translated into French

May 31, 2024

NextSeq 1000/2000 Product Documentation Translated into German

May 31, 2024

NextSeq 1000/2000 Product Documentation Translated into Greek

May 31, 2024

NextSeq 1000/2000 Product Documentation Translated into Hebrew

May 31, 2024

NextSeq 1000/2000 Product Documentation Translated into Italian

May 31, 2024

NextSeq 1000/2000 Product Documentation Translated into Japanese

Oct 11, 2024

NextSeq 1000/2000 Product Documentation Translated into Korean

Jul 22, 2024

NextSeq 1000/2000 Product Documentation Translated into Latvian

May 31, 2024

NextSeq 1000/2000 Product Documentation Translated into Lithuanian

May 31, 2024

NextSeq 1000/2000 Product Documentation Translated into Norwegian

May 31, 2024

NextSeq 1000/2000 Product Documentation Translated into Polish

May 31, 2024

NextSeq 1000/2000 Product Documentation Translated into Portuguese

May 31, 2024

NextSeq 1000/2000 Product Documentation Translated into Simplified Chinese

May 31, 2024

NextSeq 1000/2000 Product Documentation Translated into Slovak

May 31, 2024

NextSeq 1000/2000 Product Documentation Translated into Swedish

May 31, 2024

NextSeq 1000/2000 Product Documentation Translated into Traditional Chinese

Jun 3, 2024

NextSeq 1000/2000 Product Documentation Translated into Turkish

May 31, 2024

NextSeq 1000/2000 Product Documentation Translated into Ukrainian

May 31, 2024

NextSeq 1000/2000 Product Documentation Translated into Vietnamese

May 31, 2024

Denature and Dilute Protocol Generator

Instructions for denaturing and diluting prepared libraries for sequencing.

Generate Protocol

Feb 28, 2025

RFID Reader Compliance Guide

Safety considerations, compliance markings, and regulatory statements for the RFID reader module, an internal instrument component.

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RFID Reader Module Compliance Guide (1000000002699 v05)

< 1 MB

Apr 13, 2020

RFID Reader Module Compliance Guide in Arabic

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Armenian

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Brazilian Portuguese

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Bulgarian

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Simplified Chinese

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Traditional Chinese

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Czech

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Danish

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Dutch

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Estonian

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Finnish

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in French

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in German

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Greek

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Hebrew

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Hungarian

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Italian

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Japanese

< 1 MB

Oct 15, 2020

RFID Reader Module Compliance Guide in Korean

< 1 MB

Nov 3, 2020

RFID Reader Module Compliance Guide in Latvian

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Lithuanian

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Maltese

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Norwegian

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Polish

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Portuguese

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Russian

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Slovak

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Slovenian

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Spanish

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Swedish

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Turkish

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Ukrainian

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Vietnamese

< 1 MB

May 26, 2020

Supporting Documents

SIZE

DATE

Indexed Sequencing Overview

The sequencing process for single- and dual-indexed runs on Illumina instruments.

View Online Help

Aug 13, 2024

Security and Networking

Guidelines for managing the security of the control computer on your sequencing system. Use these guidelines to configure your system and ensure a more secure operating environment.

It is strongly recommended that you review this documentation to keep your instrument secure.

This site is updated frequently. Make sure to visit this site often for the latest security recommendations.

View Online Help

Product Literature	TYPE	SIZE	DATE
Data concordance between the NextSeq 1000/2000 and NextSeq 550 Sequencing Systems Download	Application note	< 1 MB	Mar 23, 2020
Methods for RNA sequencing Download	ebooks	2 MB	May 6, 2021
Illumina Sequencing Platforms Brochure Download	Brochure	1 MB	Oct 9, 2024
DRAGEN Bio-IT Platform Onboard NextSeq 1000 and NextSeq 2000 Systems Download	Data sheet	< 1 MB	Aug 2, 2022
NextSeq 1000 and NextSeq 2000 Sequencing Systems specification sheet Download	Data sheet	15 MB
Explore the transcriptome with single-cell resolution Download	Technical note	1 MB	Mar 1, 2022
Data Security with Illumina Proactive Download	Technical note	< 1 MB	Oct 4, 2022
Multiomic interrogation of the immune system at single-cell resolution Download	Technical note	1 MB	Mar 1, 2022
Unify single-cell gene expression and chromatin accessibility Download	Technical note	1 MB
Resolve the whole transcriptome within tissue architecture Download	Technical note	3 MB	Jul 1, 2021
Superior performance with the NextSeq 2000 System Download	Technical note	< 1 MB
XLEAP-SBS chemistry expands NextSeq 1000 and NextSeq 2000 System capabilities Download	Technical note	< 1 MB	May 30, 2024
Full-length V(D)J immune repertoire sequencing (IR-Seq) on the NextSeq™ 1000 and NextSeq 2000 Systems Download	Application note	< 1 MB
16S rRNA sequencing on NextSeq™ 1000 and NextSeq 2000 Systems Download	Application note	1 MB
High-plex spatial proteogenomics of FFPE tissue sections Download	Application note	2 MB
NextSeq™ 1000 and NextSeq 2000 single-cell RNA sequencing solution Download	Application note	< 1 MB	May 30, 2024
NextSeq 1000 and NextSeq 2000 RNA sequencing solution Download	Application note	< 1 MB	May 30, 2024
A shotgun metagenomics NGS workflow for assessing microbial populations in complex samples Download	Application note	< 1 MB
High-resolution, high-throughput spatial transcriptomics of complex tissues Download	Application note	3 MB	Aug 24, 2021
Small whole-genome sequencing on NextSeq 1000 and NextSeq 2000 Systems Download	Application note	< 1 MB
Single-cell and spatial sequencing on NextSeq 1000 and NextSeq 2000 XLEAP-SBS flow cells Download	Application note	2 MB	Nov 22, 2024
NextSeq 1000 and NextSeq 2000 exome sequencing solution Download	Application note	< 1 MB

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Next-generation sequencing for beginners

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NextSeq 1000/2000 Sequencing System Documentation

NextSeq 1000/2000 Sequencing System

Documentation

System Documents

SIZE

DATE

NextSeq 1000/2000 Product Documentation

Denature and Dilute Protocol Generator

RFID Reader Compliance Guide

Supporting Documents

SIZE

DATE

Indexed Sequencing Overview

Security and Networking

Product Literature

TYPE

SIZE