Before preparing libraries, record information about your samples using an option available for your sequencing system:
The AmpliSeq for Illumina BRCA Panel is a targeted resequencing assay for research of somatic and germline variants across BRCA1 and BRCA2 genes. For detailed specifications, see AmpliSeq for Illumina BRCA Panel.
| Number of Pools | Concentration | Number of Amplicons | Average Amplicon Length (bp) | Average Library Length (bp) |
|---|---|---|---|---|
| 1 | 5X | 265 | 98 | 238 |
Completing the AmpliSeq for Illumina BRCA protocol requires the panel and the following library prep kit and index kit. The AmpliSeq for Illumina Library PLUS Kit is available in 24-, 96-, and 384-reaction configurations.
For each DNA sample, the AmpliSeq for Illumina BRCA Panel generates one DNA library. A sample is a specimen of nucleic acid from one source, while a library is nucleic acids from a sample after preparation. A library has the ends required for sequencing.
Preparing the appropriate number of samples requires the following number and combination of kits.
| Number of Samples | 24 Samples | 96 Samples | 384 Samples |
|---|---|---|---|
| Number of Libraries | 24 Libraries | 96 Libraries | 384 Libraries |
| AmpliSeq for Illumina BRCA Panel | 1 | 4 | 16 |
| AmpliSeq Library PLUS For Illumina | 1 24-reaction kit | 1 96-reaction kit | 1 384-reaction kit |
| AmpliSeq CD Set A (96 reaction, 96 indexes) | 1 | 1 | 4 |
| cDNA Synthesis | Not applicable | Not applicable | Not applicable |
Sequence AmpliSeq for Illumina BRCA libraries using the following systems and reagent kits.
| System | Reagent Kit | Libraries per Run |
|---|---|---|
| MiSeq System | MiSeq Reagent Nano Kit v2 | 32 |
| MiSeq Reagent Micro Kit v2 | 96 |
| System | Reagent Kit | Libraries per Run |
|---|---|---|
| MiniSeq System | MiniSeq Mid Output Reagent Kit | 24 |
| MiniSeq High Output Reagent Kit | 80 | |
| MiSeq System | MiSeq Reagent Nano Kit v2 | 3 |
| MiSeq Reagent Micro Kit v2 | 12 | |
| MiSeq Reagent Kit v2 | 48 | |
| MiSeq Reagent Kit v3 | 80 |