AmpliSeq for Illumina BRCA Panel Compatible Products

Sample Information

Before preparing libraries, record information about your samples using an option available for your sequencing system:

Library Prep

Panel Description

The AmpliSeq for Illumina BRCA Panel is a targeted resequencing assay for research of somatic and germline variants across BRCA1 and BRCA2 genes. For detailed specifications, see AmpliSeq for Illumina BRCA Panel.

Number of Pools Concentration Number of Amplicons Average Amplicon Length (bp) Average Library Length (bp)
1 5X 265 98 238
Library Prep and Index Kits

Completing the AmpliSeq for Illumina BRCA protocol requires the panel and the following library prep kit and index kit. The AmpliSeq for Illumina Library PLUS Kit is available in 24-, 96-, and 384-reaction configurations.

Number of Samples

For each DNA sample, the AmpliSeq for Illumina BRCA Panel generates one DNA library. A sample is a specimen of nucleic acid from one source, while a library is nucleic acids from a sample after preparation. A library has the ends required for sequencing.

Preparing the appropriate number of samples requires the following number and combination of kits.

Number of Samples 24 Samples 96 Samples 384 Samples
Number of Libraries 24 Libraries 96 Libraries 384 Libraries
AmpliSeq for Illumina BRCA Panel 1 4 16
AmpliSeq Library PLUS For Illumina 1 24-reaction kit 1 96-reaction kit 1 384-reaction kit
AmpliSeq CD Set A (96 reaction, 96 indexes) 1 1 4
cDNA Synthesis Not applicable Not applicable Not applicable

Sequencing Guidelines

Sequence AmpliSeq for Illumina BRCA libraries using the following systems and reagent kits.

Germline Variants
System Reagent Kit Libraries per Run
MiSeq System MiSeq Reagent Nano Kit v2 32
  MiSeq Reagent Micro Kit v2 96
Somatic Variants
System Reagent Kit Libraries per Run
MiniSeq System MiniSeq Mid Output Reagent Kit 24
  MiniSeq High Output Reagent Kit 80
MiSeq System MiSeq Reagent Nano Kit v2 3
  MiSeq Reagent Micro Kit v2 12
  MiSeq Reagent Kit v2 48
  MiSeq Reagent Kit v3 80