AmpliSeq for Illumina Custom and Community Panels Training

Recorded Webinar (November 2021) | This webinar introduces our line of amplicon products: AmpliSeq for Illumina- compatible with Illumina next-generation sequencing platforms. We will discuss the following topics: available fixed panels, library preparation workflow, the new Design Studio, and and overview of analysis options.

This course identifies the items needed for the AmpliSeq for Illumina library prep protocol, introduces pool planning resources for a multiplexed amplicon run, demonstrates the steps in the library prep protocol, and lists best practices for the protocol.

This course describes the AmpliSeq for Illumina assay technology, lists the three types of panels, introduces the steps in the amplicon sequencing workflow, and lists the analysis options for amplicon sequencing data.

Recorded Webinar (March 2020) | Have a targeted sequencing project in mind? This webinar is targeted toward new and intermediate users looking to design a custom targeted sequencing assay in DesignStudio. We will show you how to use the software, and share tips and tricks to create designs for AmpliSeq for Illumina, Nextera Rapid Capture, and TruSeq Targeted RNA Expression.

What are the impacts of sequencing amplicon and low diversity libraries on Illumina instruments? An Illumina Field Applications Scientist discusses considerations for designing and performing amplicon sequencing.

An Illumina Field Applications Scientist discusses best practices for sequencing amplicons on Illumina instruments with high-quality results. We use Sequencing Analysis Viewer (SAV) to compare key metrics of amplicon sequencing runs to a standard PhiX run.

Recorded Webinar (October 2021) | Does my library look good to sequence? What’s that peak? This webinar will address how to use the Agilent BioAnalyzer to check library quality prior to sequencing, and troubleshoot sample preparation. This webinar is geared toward new and intermediate users, and will cover how to identify features of an ideal library trace, recognize potential issues, and prevent potential issues.

Many next-generation sequencing applications use the Polymerase Chain Reaction, or PCR, for DNA amplification. This video presents best practices to minimize the potential for PCR contamination in your experiment.