Before preparing libraries, record information about your samples using an option available for your sequencing system:
The AmpliSeq for Illumina Immune Response Panel is a targeted resequencing assay for quantitating expression of cancer biomarkers in 395 genes involved in tumor-immune system interactions.
| Number of Pools | Concentration | Number of Amplicons | Average Amplicon Length (bp) | Average Library Length (bp) |
|---|---|---|---|---|
| 1 | 5X | 398 | 106 | 246 |
Completing the AmpliSeq for Illumina Immune Response Panel protocol requires the panel and the following library prep kit and index kit.
For each RNA sample, AmpliSeq for Illumina Immune Response Panel generates one RNA library. A sample is a specimen of nucleic acid from one source, while a library is nucleic acids from a sample after preparation. A library has the ends required for sequencing.
Preparing the appropriate number of samples requires the following number and combination of kits. The AmpliSeq for Illumina Library PLUS Kit is available in 24-, 96-, and 384-reaction configurations.
| Number of Samples | 24 Samples | 96 Samples | 384 Samples |
|---|---|---|---|
| Number of Libraries | 24 Libraries | 96 Libraries | 384 Libraries |
| AmpliSeq for Illumina Immune Response Panel | 1 | 4 | 16 |
| AmpliSeq Library PLUS For Illumina | 1 24-reaction kit | 1 96-reaction kit | 1 384-reaction kit |
| AmpliSeq CD Set A (96 reaction, 96 indexes) | 1 | 1 | 4 |
| cDNA Synthesis | 1 | 1 | 4 |
Sequence AmpliSeq for Illumina Immune Response Panel libraries using the following systems and reagent kits:
| System | Reagent Kit | Samples per Run |
|---|---|---|
| MiniSeq System | MiniSeq Mid Output Reagent Kit | 8 |
| MiniSeq High Output Reagent Kit | 24 | |
| MiSeq System | MiSeq Reagent Micro Kit v2 | 4 |
| MiSeq Reagent Kit v2 | 16 | |
| MiSeq Reagent Kit v3 | 24 | |
| NextSeq System | NextSeq Mid Output v2 Kit | 96 |