This course introduces the library prep kit, identifies the required items, and defines the various steps in the library preparation and enrichment workflows.
Recorded Webinar (August 2021) | Part 1 of a 3-Part RNA-Seq Webinar Series. This session will review sample preparation kit options for RNA input. An introduction to the new Illumina Stranded mRNA Ligation, Illumina Stranded Total RNA Ligation with Ribozero Plus, and Illumina RNA prep with Enrichment Tagmentation workflows will be provided along with a comparison to established Illumina kits. This webinar is targeted towards both new and experienced users and will cover the following topics: key considerations when planning RNA sequencing experiments, how to identify which kit is right for your experiment, what are the expected input and outputs, and where to find additional resources.
Recorded Webinar (September 2021) | In the second part of our RNA-Seq Webinar series, we dive into best practices for Illumina’s RNA kits. This webinar is aimed at both new and experienced users with a basic understanding of Illumina Sequencing technology and workflow. We will focus on TruSeq RNA v2, TruSeq Stranded mRNA, Illumina Stranded mRNA Ligation, TruSeq Stranded Total RNA, Illumina Stranded Total RNA Ligation and Illumina RNA prep with Enrichment Tagmentation. We will cover the following topics: bead handling, quantification methods, library QC and troubleshooting.
Recorded Webinar (August 2019) | Part 3 of a 3-Part RNA-Seq Webinar Series. In this webinar, we will discuss the RNA-Seq analysis options available on BaseSpace including the RNA-Seq Alignment/Cufflinks, TopHat/Cufflinks, and RNA Express apps. We assume both a basic understanding of Illumina sequencing technology and knowledge of the general library prep workflow for the TruSeq RNA kits. The webinar is targeted towards both new and experienced users. Please note that small RNA sequencing analysis is not be covered in this webinar. We encourage you to view Part I: Introduction to Illumina’s RNA library preparation workflows and Part II: Best practices for Illumina’s RNA Prep protocols, prior to this webinar.
Upcoming Webinar | Join Illumina Technical Support to learn about the new Illumina RNA Prep with Enrichment workflow. This webinar is intended for users that are interested in targeted RNA sequencing using enrichment with fixed and custom panels. We will discuss the following topics: sample to library workflow, unique features of Illumina RNA Prep with Enrichment and useful tips for a successful library prep.
Jun 07, 2022 PST