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Illumina Stranded mRNA Prep Ligation Contents & Storage

Illumina Stranded mRNA Prep, Ligation (16 Samples) (20040532)

Illumina PolyA Capture
Quantity Reagent Description Storage
1 BBB Bead Binding Buffer 2°C to 8°C
1 BWB Bead Washing Buffer 2°C to 8°C
3 ELB Elution Buffer 2°C to 8°C
1 RPBX RNA Purification Beads 2°C to 8°C
Illumina cDNA Synthesis
Quantity Reagent Description Storage
1 EPH3 Elute, Prime, Fragment, High Concentration Mix -25°C to -15°C
1 FSA Bead Washing Buffer -25°C to -15°C
1 RSB Elution Buffer -25°C to -15°C
1 RVT Reverse Transcriptase -25°C to -15°C
1 SMM Second Strand Marking Master Mix -25°C to -15°C
Illumina RNA Prep, Ligation
Quantity Reagent Description Storage
1 ATL4 A-Tailing Mix -25°C to -15°C
1 EPM Enhanced PCR Mix -25°C to -15°C
1 LIGX Ligation Mix -25°C to -15°C
1 RSB Resuspension Buffer -25°C to -15°C
1 STL Stop Ligation Buffer -25°C to -15°C

Illumina Stranded mRNA Prep, Ligation (96 Samples) (20040534)

Illumina PolyA Capture
Quantity Reagent Description Storage
1 BBB Bead Binding Buffer 2°C to 8°C
1 BWB Bead Washing Buffer 2°C to 8°C
3 ELB Elution Buffer 2°C to 8°C
4 RPBX RNA Purification Beads 2°C to 8°C
Illumina cDNA Synthesis
Quantity Reagent Description Storage
4 EPH3 Elute, Prime, Fragment, High Concentration Mix -25°C to -15°C
4 FSA Bead Washing Buffer -25°C to -15°C
2 RSB Elution Buffer -25°C to -15°C
1 RVT Reverse Transcriptase -25°C to -15°C
4 SMM Second Strand Marking Master Mix -25°C to -15°C
Illumina RNA Prep, Ligation
Quantity Reagent Description Storage
1 ATL4 A-Tailing Mix -25°C to -15°C
1 EPM Enhanced PCR Mix -25°C to -15°C
1 LIGX Ligation Mix -25°C to -15°C
1 RSB Resuspension Buffer -25°C to -15°C
1 STL Stop Ligation Buffer -25°C to -15°C

IDT for Illumina RNA UD Indexes Set A Ligation (96 Indexes, 96 Samples) (20040553)

Quantity Reagent Description
1 IDT for Illumina RNA Index Anchors -25°C to -15°C
1 IDT for Illumina DNA/RNA UD Indexes Set A (UDP0001–UDP0096) -25°C to -15°C

IDT for Illumina RNA UD Indexes Set B Ligation (96 Indexes, 96 Samples) (20040554)

Quantity Reagent Description
1 IDT for Illumina RNA Index Anchors -25°C to -15°C
1 IDT for Illumina DNA/RNA UD Indexes Set B (UDP0097–UDP0192) -25°C to -15°C

IDT for Illumina RNA UD Indexes Set C Ligation (96 Indexes, 96 Samples) (2004555)

Quantity Reagent Description
1 IDT for Illumina RNA Index Anchors -25°C to -15°C
1 IDT for Illumina DNA/RNA UD Indexes Set C (UDP0193–UDP0288) -25°C to -15°C

IDT for Illumina RNA UD Indexes Set D Ligation (96 Indexes, 96 Samples) (20040556)

Quantity Reagent Description
1 IDT for Illumina RNA Index Anchors -25°C to -15°C
1 IDT for Illumina DNA/RNA UD Indexes Set D (UDP0289–UDP0384) -25°C to -15°C
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