Mate Pair Library Prep Kit v2 Contents & Storage

Box 1: Store at -15° to -25°C

Reagent Name

Storage

Part Number

Ultra Pure Water

-15° to -25°C

1001913

End Repair Buffer

-15° to -25°C

1006578

Natural dNTP Mix

-15° to -25°C

15007985

T4 DNA Polymerase

-15° to -25°C

15008323

T4 Poly Nucleotide Kinase

-15° to -25°C

15008327

Klenow DNA Polymerase

-15° to -25°C

15008325

Biotin dNTP Mix

-15° to -25°C

15008011

Circularization Buffer 10X

-15° to -25°C

1006640

Circularization Ligase

-15° to -25°C

15008331

DNA Exonuclease

-15° to -25°C

15008329

A-Tailing Buffer 10X

-15° to -25°C

1006579

1 mM dATP

-15° to -25°C

11318081

A-Tailing Enzyme

-15° to -25°C

1006679

Adapter Ligation Buffer 2X

-15° to -25°C

1006574

PE Adapter Oligo Mix (15 µM)

-15° to -25°C

1006582

Adapter Ligase

-15° to -25°C

1006573

Phusion* DNA Polymerase

-15° to -25°C

1000524

PCR Primer PE 1.0

-15° to -25°C

1001783

PCR Primer PE 2.0

-15° to -25°C

1001784

*Phusion is a trademark of Finnzymes Oy.

Box 2: Store at Room Temperature

Reagent

Storage

Part Number

Streptavidin Bead Binding Buffer

Room Temperature

1006643

Streptavidin Bead Wash Buffer 2

Room Temperature

1006645

EDTA (0.5 M)

Room Temperature

15008333

Streptavidin Bead Wash Buffer 1

Room Temperature

1006644