Before preparing libraries, create a sample sheet to record the index adapter combinations and other information about your samples. Sample sheet options and requirements depend on the instrument. For information, see the instrument support pages.
Nextera XT Indexes (24 indexes, 96 samples) are compatible with the Nextera DNA XT Library Prep Kit and bead-based normalization. For standard normalization, use the IDT for Illumina Nextera DNA UD Indexes.
The Nextera XT Indexes (96 indexes, 384 samples) (catalog # FC-131-1002) was obsolesced with the introduction of the Nextera XT Index Kit v2.