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TruSeq Methyl Capture Library Prep Kit Contents & Storage

TruSeq Methyl Capture EPIC LT Kit

Box 1, Bisulphite Conversion Reagents, Store at Room Temperature
Quantity Reagent
1 Lightning Conversion Reagent
1 M-Binding Buffer
1 MagBinding Beads
1 M-Wash Buffer
1 L-Desulphonation Buffer
Box 2, SPB Reagents, Store at 2°C to 8°C
Quantity Reagent Description
1 SMB Streptavidin Magnetic Beads
2 SPB Sample Purification Beads
1 EHB2 Enrichment Hybridization Buffer 2
1 ET2 Elute Target Buffer 2
1 SPM Sample Purification Mix
Box 3, Adapters, Store at -25°C to -15°C
Quantity Reagent Description
1 AD002 DNA Adapter Index 2
2 AD004 DNA Adapter Index 4
1 AD005 DNA Adapter Index 5
1 AD006 DNA Adapter Index 6
Box 4, Core Reagents, Store at -25°C to -15°C
Quantity Reagent Description
1 ERP3 End Repair Mix 3
1 ATL2 A-Tailing Mix
1 LIG2 Ligation Mix 2
1 RSB Resuspension Buffer
1 STL Stop Ligation Buffer
1 CT3 Capture Target Buffer 3
1 CT4 Capture Target Buffer 4
1 EWS Enrichment Wash Solution
1 BLR Blocker
1 HP3 2 N NaOH
1 ET1 Elute Target Buffer 1
1 PPC PCR Primer Cocktail
1 EDtA 0.5 M EDTA
Box 5, Probes, Store at -25°C to -15°C
Quantity Reagent Description
1 EPIC Epigenetic Oligo Pool

TruSeq Methyl Capture EPIC HT Kit

Box 1, Bisulphite Conversion Reagents, Store at Room Temperature
Quantity Reagent
1 Lightning Conversion Reagent
1 M-Binding Buffer
1 MagBinding Beads
1 M-Wash Buffer
1 L-Desulphonation Buffer
Box 2, SPB Reagents, Store at 2°C to 8°C
Quantity Reagent Description
3 SMB Streptavidin Magnetic Beads
1 SPB Sample Purification Beads
1 EHB2 Enrichment Hybridization Buffer 2
1 ET2 Elute Target Buffer 2
1 SPM Sample Purification Mix
Box 3, Adapters, Store at -25°C to -15°C
Quantity Reagent Description
1 AD002 DNA Adapter Index 2
2 AD004 DNA Adapter Index 4
1 AD005 DNA Adapter Index 5
1 AD006 DNA Adapter Index 6
1 AD007 DNA Adapter Index 7
1 AD012 DNA Adapter Index 12
1 AD013 DNA Adapter Index 13
1 AD014 DNA Adapter Index 14
1 AD015 DNA Adapter Index 15
1 AD016 DNA Adapter Index 16
1 AD018 DNA Adapter Index 18
1 AD019 DNA Adapter Index 19
Box 4, Core Reagents, Store at -25°C to -15°C
Quantity Reagent Description
1 ERP3 End Repair Mix 3
1 ATL2 A-Tailing Mix
1 LIG2 Ligation Mix 2
1 RSB Resuspension Buffer
1 STL Stop Ligation Buffer
2 CT3 Capture Target Buffer 3
1 CT4 Capture Target Buffer 4
1 EWS Enrichment Wash Solution
1 BLR Blocker
1 HP3 2 N NaOH
3 ET1 Elute Target Buffer 1
1 PPC PCR Primer Cocktail
1 EDtA 0.5 M EDTA
Box 5, Probes, Store at -25°C to -15°C
Quantity Reagent Description
4 EPIC Epigenetic Oligo Pool
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