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TruSeq Stranded Total RNA Contents & Storage

TruSeq Stranded Total RNA Library Prep (48 Samples))

Library Prep Box 1
Quantity Reagent Description Storage
1 DTE CTE Dilution Tube Room temperature
1 DTA CTA Dilution Tube Room temperature
1 DTL CTL Dilution Tube Room temperature
1 RRB rRNA Removal Beads 2°C to 8°C
Library Prep Ribo-Zero Box

You will receive one of the following boxes, depending on the workflow. The box also contains plate barcode labels.

Ribo-Zero H/M/R
Quantity Reagent Description Storage
1 RBB rRNA Binding Buffer -25°C to -15°C
1 RRM rRNA Removal Mix -25°C to -15°C
1 ELB Elution Buffer 2°C to 8°C
1 EPH Elute, Prime, Fragment High Mix -25°C to -15°C
Ribo-Zero Gold
Quantity Reagent Description Storage
1 RBB rRNA Binding Buffer -25°C to -15°C
1 RRM G rRNA Removal Mix - Gold -25°C to -15°C
1 ELB Elution Buffer 2°C to 8°C
1 EPH Elute, Prime, Fragment High Mix -25°C to -15°C
Ribo-Zero Plant
Quantity Reagent Description Storage
1 RBB rRNA Binding Buffer -25°C to -15°C
1 RRM P rRNA Removal Mix - Plant -25°C to -15°C
1 ELB Elution Buffer 2°C to 8°C
1 EPH Elute, Prime, Fragment High Mix -25°C to -15°C
Ribo-Zero Globin
Quantity Reagent Description Storage
1 RBB rRNA Binding Buffer -25°C to -15°C
1 GRM Globin Removal Mix -25°C to -15°C
1 ELB Elution Buffer 2°C to 8°C
1 EPH Elute, Prime, Fragment High Mix -25°C to -15°C
Quantity Reagent Description
1 ATL A-Tailing Mix
1 CTA A-Tailing Control
1 CTE End Repair Control
1 CTL Ligation Control
1 LIG Ligation Mix
1 RSB Resuspension Buffer
1 STL Stop Ligation Buffer
Core Library Prep Box 2, Store at -25°C to -15°C
Quantity Reagent Description
1 PMM PCR Master Mix
1 PPC PCR Primer Cocktail
1 FSA First Strand Synthesis Act D Mix
1 SMM Second Strand Marker Master Mix

TruSeq Stranded Total RNA Library Prep (96 Samples))

Library Prep Box 1
Quantity Reagent Description Storage
1 DTE CTE Dilution Tube Room temperature
1 DTA CTA Dilution Tube Room temperature
1 DTL CTL Dilution Tube Room temperature
2 RRB rRNA Removal Beads 2°C to 8°C
Library Prep Ribo-Zero Box

You will receive two of one of the following boxes, depending on the workflow. The box also contains plate barcode labels.

Library Preparation H/M/R (48 Samples)
Quantity Reagent Description Storage
2 RBB rRNA Binding Buffer -25°C to -15°C
2 RRM rRNA Removal Mix -25°C to -15°C
2 ELB Elution Buffer 2°C to 8°C
2 EPH Elute, Prime, Fragment High Mix -25°C to -15°C
Library Preparation Gold (48 Samples)
Quantity Reagent Description Storage
2 RBB rRNA Binding Buffer -25°C to -15°C
2 RRM G rRNA Removal Mix - Gold -25°C to -15°C
2 ELB Elution Buffer 2°C to 8°C
2 EPH Elute, Prime, Fragment High Mix -25°C to -15°C
Library Preparation Plant (48 Samples)
Quantity Reagent Description Storage
2 RBB rRNA Binding Buffer -25°C to -15°C
2 RRM P rRNA Removal Mix - Plant -25°C to -15°C
2 ELB Elution Buffer 2°C to 8°C
2 EPH Elute, Prime, Fragment High Mix -25°C to -15°C
Library Preparation Globin (48 Samples)
Quantity Reagent Description Storage
2 RBB rRNA Binding Buffer -25°C to -15°C
2 GRM Globin Removal Mix -25°C to -15°C
2 ELB Elution Buffer 2°C to 8°C
2 EPH Elute, Prime, Fragment High Mix -25°C to -15°C
Quantity Reagent Description
2 ATL A-Tailing Mix
2 CTA A-Tailing Control
2 CTE End Repair Control
2 CTL Ligation Control
2 LIG Ligation Mix
2 RSB Resuspension Buffer
2 STL Stop Ligation Buffer
Core Library Prep Box 2, Store at -25°C to -15°C
Quantity Reagent Description
2 PMM PCR Master Mix
2 PPC PCR Primer Cocktail
2 FSA First Strand Synthesis Act D Mix
2 SMM Second Strand Marker Master Mix
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