The TruSight Cardio Sequencing Kits contain the following components and is shipped on dry ice. Store the kit components at the specified temperature in designated pre-amplification and post-amplification areas.
TruSight Cardio Sequencing Kit Contents for MiSeq, v2 Chemistry (12 Samples)
Box 1, Store at 2°C to 8°C
| Quantity | 
Reagent | 
Description | 
| 1 | 
SPB | 
Sample Purification Beads | 
| 1 | 
SMB | 
Streptavidin Magnetic Beads | 
| 1 | 
ET2 | 
Elute Target Buffer 2 | 
| 1 | 
ST | 
Stop Tagment Buffer | 
 
Box 2, Store at -25°C to -15°C
| Quantiy | 
Reagent | 
Description | 
| 1 | 
TDE1 | 
Tagment DNA Enzyme | 
| 1 | 
EE1 | 
Enrichment Elution Buffer 1 | 
| 1 | 
TD | 
Tagment DNA Buffer | 
| 1 | 
RSB | 
Resuspension Buffer | 
| 1 | 
NLM | 
Nextera Amplification Mix | 
| 1 | 
EHB | 
Enrichment Hybridization Buffer | 
| 1 | 
EWS | 
Enrichment Wash Solution | 
| 1 | 
HP3 | 
2N NaOH | 
| 1 | 
PPC | 
PCR Primer Cocktail | 
| 1 | 
NEM | 
Nextera Enrichment Amplification Mix | 
 
Box 3 Indexes, Store at -25°C to -15°C
| Quantity | 
Reagent | 
Description | 
| 1 | 
Index Primer | 
E505 | 
| 12 | 
Index Primers | 
N701 to N712 | 
 
Box 4 Oligos, Store at -25°C to -15°C
| Quantity | 
Description | 
| 1 | 
TruSight Cardio Oligos | 
 
Box 5, PhiX Control v3, Store at -25°C to -15°C
| Quantity | 
Description | 
| 1 | 
PhiX v3 | 
 
MiSeq Reagent Kit v2 Box 1, Store at -25°C to -15°C
| Quantity | 
Description | 
| 1 | 
Hybridization Buffer | 
| 1 | 
MiSeq v2 Cartridge, 300 cycles | 
 
MiSeq Reagent Kit v2 Box 2, Store at 2°C to 8°C
| Quantity | 
Description | 
| 1 | 
MiSeq Flow Cell | 
| 1 | 
PR2 Bottle | 
 
 
TruSight Cardio Sequencing Kit Contents for NextSeq, Mid Output (48 Samples)
Box 1 TruSight Rapid Capture Reagents, Store at 2°C to 8°C
| Quantity | 
Reagent | 
Description | 
| 2 | 
SPB | 
Sample Purification Beads | 
| 2 | 
SMB | 
Streptavidin Magnetic Beads | 
| 1 | 
ET2 | 
Elute Target Buffer 2 | 
| 1 | 
ST | 
Stop Tagment Buffer | 
 
Box 2 TruSight Rapid Capture Reagents, Store at -25°C to -15°C
| Quantiy | 
Reagent | 
Description | 
| 2 | 
TDE1 | 
Tagment DNA Enzyme | 
| 1 | 
EE1 | 
Enrichment Elution Buffer 1 | 
| 1 | 
TD | 
Tagment DNA Buffer | 
| 1 | 
RSB | 
Resuspension Buffer | 
| 2 | 
NLM | 
Nextera Amplification Mix | 
| 1 | 
EHB | 
Enrichment Hybridization Buffer | 
| 1 | 
EWS | 
Enrichment Wash Solution | 
| 1 | 
HP3 | 
2N NaOH | 
| 1 | 
PPC | 
PCR Primer Cocktail | 
| 2 | 
NEM | 
Nextera Enrichment Amplification Mix | 
 
Box 3 Indexes, Store at -25°C to -15°C
| Quantity | 
Reagent | 
Description | 
| 4 | 
Index Primer | 
E502 to E506 | 
| 12 | 
Index Primers | 
N701 to N712 | 
 
Box 4 Oligos, Store at -25°C to -15°C
| Quantity | 
Description | 
| 4 | 
TruSight Cardio Oligos | 
 
Box 5, PhiX Control v3, Store at -25°C to -15°C
| Quantity | 
Description | 
| 1 | 
PhiX v3 | 
 
NextSeq Reagents
| Quantity | 
Description | 
Storage | 
| 1 | 
NextSeq 500 Mid Output Reagent Cartridge v2 (300 cycles) | 
-25°C to -15°C | 
| 1 | 
NextSeq 500 Mid Output Flow Cell | 
2°C to 8°C | 
| 1 | 
Hybridization Buffer | 
Room temperature |