TruSight Oncology High Throughput Contents & Storage

TruSight Oncology 500 High Throughput Kits

24 Samples

48 Samples

72 Samples

144 Samples

TruSight Oncology 500 DNA/RNA High-Throughput (24 Samples)

RNA Library Prep (Pre-Amp) (REF 20007000)

Quantity	Reagent	Description	Storage
1	EPH3	Elution, Primer, Fragmentation Mix	-25°C to -15°C
1	FSM	First Strand Synthesis Mix	-25°C to -15°C
1	RT	Reverse Transcriptase	-25°C to -15°C
1	SSM	Second Strand Mix	-25°C to -15°C

Library Prep without SUA (Pre-Amp) (REF 20039147)

Quantity	Reagent	Description	Storage
2	ALB1	Adapter Ligation Buffer 1	-25°C to -15°C
2	EPM	Enhanced PCR Mix	-25°C to -15°C
2	ERA1-A	End Repair A-tailing Enzyme Mix 1	-25°C to -15°C
2	ERA1-B	End Repair A-tailing Buffer 1	-25°C to -15°C
2	ALB1	Adapter Ligation Buffer 1	-25°C to -15°C
2	LIG3	DNA Ligase 3	-25°C to -15°C
2	STL	Stop Ligation Buffer	-25°C to -15°C

Library Prep (Pre-Amp) (REF 20007003)

Quantity	Reagent	Description	Storage
1	RSB	Resuspension Buffer	2°C to 8°C or -25°C to -15°C
2	SPB	Sample Purification	2°C to 8°C
1	TEB	TE Buffer	2°C to 8°C

Enrichment without TCB (Post-Amp) (20040235)

Quantity	Reagent	Description	Storage
2	ET2	Elute Target Buffer 2	2°C to 8°C
2	HP3	2N NaOH	2°C to 8°C
1	LNB1	Library Normalization Beads 1	2°C to 8°C
2	LNS1	Library Normalization Storage Buffer 1	15°C to 30°C
2	LNW1	Library Normalization Wash 1	2°C to 8°C
1	RSB	Resuspension Buffer	2°C to 8°C or -25°C to -15°C
2	SMB	Streptavidin Magnetic Beads	2°C to 8°C
2	SPB	Sample Purification Beads	2°C to 8°C

Enrichment with EHB (Post-Amp) (REF 20040234)

Quantity	Reagent	Description	Storage
3	EE2	Enrichment Elution 2	-25°C to -15°C
1	EEW2	Enhanced Enrichment Wash	-25°C to -15°C
6	EHB	Enrichment Hybridization Buffer	-25°C to -15°C
2	EPM	Enhanced PCR Mix	-25°C to -15°C
1	LNA1	Library Normalization Additives 1	-25°C to -15°C
2	PPC3	PCR Primer Cocktail 3	-25°C to -15°C

DNA Probes (Post-Amp) (REF 20026138)

Quantity	Reagent	Description	Storage
1	OPD2	Oncology DNA Probes Pool 2	-25°C to -15°C

RNA Probes (Post-Amp) (REF 20007012)

Quantity	Reagent	Description	Storage
1	OPR1	Oncology RNA Probes Pool	-25°C to -15°C

TruSight Oncology 500 DNA High-Throughput (48 Samples)

Library Prep without SUA (Pre-Amp) (REF 20039147)

Quantity	Reagent	Description	Storage
2	ALB1	Adapter Ligation Buffer 1	-25°C to -15°C
2	EPM	Enhanced PCR Mix	-25°C to -15°C
2	ERA1-A	End Repair A-tailing Enzyme Mix 1	-25°C to -15°C
2	ERA1-B	End Repair A-tailing Buffer 1	-25°C to -15°C
2	ALB1	Adapter Ligation Buffer 1	-25°C to -15°C
2	LIG3	DNA Ligase 3	-25°C to -15°C
2	STL	Stop Ligation Buffer	-25°C to -15°C

Library Prep (Pre-Amp) (REF 20007003)

Quantity	Reagent	Description	Storage
1	RSB	Resuspension Buffer	2°C to 8°C or -25°C to -15°C
2	SPB	Sample Purification	2°C to 8°C
1	TEB	TE Buffer	2°C to 8°C

Enrichment without TCB (Post-Amp) (20040235)

Quantity	Reagent	Description	Storage
2	ET2	Elute Target Buffer 2	2°C to 8°C
2	HP3	2N NaOH	2°C to 8°C
1	LNB1	Library Normalization Beads 1	2°C to 8°C
2	LNS1	Library Normalization Storage Buffer 1	15°C to 30°C
2	LNW1	Library Normalization Wash 1	2°C to 8°C
1	RSB	Resuspension Buffer	2°C to 8°C or -25°C to -15°C
2	SMB	Streptavidin Magnetic Beads	2°C to 8°C
2	SPB	Sample Purification Beads	2°C to 8°C

Enrichment with EHB (Post-Amp) (REF 20040234)

Quantity	Reagent	Description	Storage
2	EE2	Enrichment Elution 2	-25°C to -15°C
1	EEW2	Enhanced Enrichment Wash	-25°C to -15°C
6	EHB	Enrichment Hybridization Buffer	-25°C to -15°C
2	EPM	Enhanced PCR Mix	-25°C to -15°C
1	LNA1	Library Normalization Additives 1	-25°C to -15°C
2	PPC3	PCR Primer Cocktail 3	-25°C to -15°C

DNA Probes (Post-Amp) (REF 20026138)

Quantity	Reagent	Description	Storage
1	OPD2	Oncology DNA Probes Pool 2	-25°C to -15°C

TruSight Oncology 500 DNA/RNA High-Throughput (72 Samples)

RNA Library Prep (Pre-Amp) (REF 20040222)

Quantity	Reagent	Description	Storage
3	EPH3	Elution, Primer, Fragmentation Mix	-25°C to -15°C
1	FSM	First Strand Synthesis Mix	-25°C to -15°C
3	RT	Reverse Transcriptase	-25°C to -15°C
3	SSM	Second Strand Mix	-25°C to -15°C

Library Prep without SUA (Pre-Amp) (REF 0040223)

Quantity	Reagent	Description	Storage
6	ALB1	Adapter Ligation Buffer 1	-25°C to -15°C
6	EPM	Enhanced PCR Mix	-25°C to -15°C
6	ERA1-A	End Repair A-tailing Enzyme Mix 1	-25°C to -15°C
6	ERA1-B	End Repair A-tailing Buffer 1	-25°C to -15°C
6	ALB1	Adapter Ligation Buffer 1	-25°C to -15°C
6	LIG3	DNA Ligase 3	-25°C to -15°C
6	STL	Stop Ligation Buffer	-25°C to -15°C

Library Prep (Pre-Amp) (REF 20040224)

Quantity	Reagent	Description	Storage
3	RSB	Resuspension Buffer	2°C to 8°C or -25°C to -15°C
6	SPB	Sample Purification	2°C to 8°C
3	TEB	TE Buffer	2°C to 8°C

Enrichment without TCB (Post-Amp) (20040225)

Quantity	Reagent	Description	Storage
6	ET2	Elute Target Buffer 2	2°C to 8°C
6	HP3	2N NaOH	2°C to 8°C
3	LNB1	Library Normalization Beads 1	2°C to 8°C
6	LNS1	Library Normalization Storage Buffer 1	15°C to 30°C
6	LNW1	Library Normalization Wash 1	2°C to 8°C
3	RSB	Resuspension Buffer	2°C to 8°C or -25°C to -15°C
6	SMB	Streptavidin Magnetic Beads	2°C to 8°C
6	SPB	Sample Purification Beads	2°C to 8°C

Enrichment with EHB (Post-Amp) (REF 20040226)

Quantity	Reagent	Description	Storage
9	EE2	Enrichment Elution 2	-25°C to -15°C
3	EEW2	Enhanced Enrichment Wash	-25°C to -15°C
18	EHB	Enrichment Hybridization Buffer	-25°C to -15°C
6	EPM	Enhanced PCR Mix	-25°C to -15°C
3	LNA1	Library Normalization Additives 1	-25°C to -15°C
6	PPC3	PCR Primer Cocktail 3	-25°C to -15°C

DNA/RNA Probes (Post-Amp) (REF 20040227)

Quantity	Reagent	Description	Storage
3	OPD2	Oncology DNA Probes Pool 2	-25°C to -15°C
3	OPR1	Oncology RNA Probes Pool 2	-25°C to -15°C

TruSight Oncology 500 DNA High-Throughput (144 Samples)

Library Prep without SUA (Pre-Amp) (REF 20040223)

Quantity	Reagent	Description	Storage
6	ALB1	Adapter Ligation Buffer 1	-25°C to -15°C
6	EPM	Enhanced PCR Mix	-25°C to -15°C
6	ERA1-A	End Repair A-tailing Enzyme Mix 1	-25°C to -15°C
6	ERA1-B	End Repair A-tailing Buffer 1	-25°C to -15°C
6	ALB1	Adapter Ligation Buffer 1	-25°C to -15°C
6	LIG3	DNA Ligase 3	-25°C to -15°C
6	STL	Stop Ligation Buffer	-25°C to -15°C

Library Prep (Pre-Amp) (REF 20040224)

Quantity	Reagent	Description	Storage
3	RSB	Resuspension Buffer	2°C to 8°C or -25°C to -15°C
6	SPB	Sample Purification	2°C to 8°C
6	TEB	TE Buffer	2°C to 8°C

Enrichment without TCB (Post-Amp) (20040225)

Quantity	Reagent	Description	Storage
6	ET2	Elute Target Buffer 2	2°C to 8°C
6	HP3	2N NaOH	2°C to 8°C
3	LNB1	Library Normalization Beads 1	2°C to 8°C
6	LNS1	Library Normalization Storage Buffer 1	15°C to 30°C
6	LNW1	Library Normalization Wash 1	2°C to 8°C
3	RSB	Resuspension Buffer	2°C to 8°C or -25°C to -15°C
6	SMB	Streptavidin Magnetic Beads	2°C to 8°C
6	SPB	Sample Purification Beads	2°C to 8°C

Enrichment with EHB (Post-Amp) (REF 20040226)

Quantity	Reagent	Description	Storage
9	EE2	Enrichment Elution 2	-25°C to -15°C
3	EEW2	Enhanced Enrichment Wash	-25°C to -15°C
18	EHB	Enrichment Hybridization Buffer	-25°C to -15°C
6	EPM	Enhanced PCR Mix	-25°C to -15°C
3	LNA1	Library Normalization Additives 1	-25°C to -15°C
6	PPC3	PCR Primer Cocktail 3	-25°C to -15°C

DNA Probes (Post-Amp) (REF 20040755)

Quantity	Reagent	Description	Storage
6	OPD2	Oncology DNA Probes Pool 2	-25°C to -15°C