An Illumina Field Applications Scientist describes what it means to sequence through the insert of your prepared library. We look at Bioanalyzer traces to assess the range of insert sizes in a library.
This video shows you how to use Sequencing Analysis Viewer (SAV) plots to determine if your sequencing insert is too short. We also explore how to use the adaptertrimming.txt file on the MiSeq to diagnose short inserts.
This video provides an overview of overclustering and how it can impact your sequencing data. An Illumina Field Applications Scientist shows you how to use Sequencing Analysis Viewer (SAV) to look for common symptoms of an overclustered HiSeq or MiSeq flow cell.
Recorded Webinar (October 2021) | Illumina Technical Support invites users to join us for a discussion of the MiSeq system. The MiSeq System offers integrated cluster generation, amplification, sequencing, and data analysis into a single instrument. This webinar will go through troubleshooting considerations at each of these steps and is targeted toward new and intermediate users with a basic understanding of the Illumina sequencing workflow and the Sequence Analysis Viewer (SAV) software. We will cover the following topics: run setup and start best practices, assessment of runs n progress using SAV, and tips and best practies
This course explores the options for monitoring your MiniSeq run. Also detailed are the key metrics in MiniSeq Control Software, Sequencing Analysis Viewer, and BaseSpace Sequence Hub.
This course introduces four options for monitoring your NextSeq 550 run. It also describes key metrics in NextSeq Control Software (NCS), Local Run Manager, Sequencing Analysis Viewer (SAV), and BaseSpace Sequence Hub.
Recorded Webinar (December 2020) | The Sequencing Analysis Viewer (SAV) Software is an application where users can view important quality metrics generated during sequencing runs. This webinar will provide a guided tour for beginners on how to use SAV, as well as tips and tricks for reviewing the most useful information for sequencing runs. This webinar is targeted at new users and will go over the following topics: how to load data into SAV, what metrics are available in each tab of the software, and understanding which are the most valuable metrics for run review and where to find them.
This course provides the basics on how to get started using Sequencing Analysis Viewer (SAV). By the end of this course, you will be able to: identify the purpose of SAV, list the steps to load data into SAV, and describe the tabs in SAV.
Recorded Webinar (January 2021) | Low diversity libraries, such as those used for amplicon sequencing, can present challenges on Illumina sequencing systems and may skew software performance and data accuracy resulting in poor quality runs. This webinar is ideal for new and intermediate users interested in considerations for the sequencing of low diversity libraries and will cover the following topics: Understanding and visualizing base diversity in Sequencing Analysis Viewer (SAV), strategies to optimize low diversity sequencing data, % PhiX spike-in and cluster density recommendations for different sequencing platforms, and an overview of base calling and quality score calculations.
This course discusses general guidelines for assessing your run’s performance.