HiSeq 1000 Reagent Kits

Kit Requirements

Performing a run requires one TruSeq Cluster Kit v3 and one TruSeq SBS Kit v3.

• The cluster kit is available in available in paired-end (PE) and single-read (SR) versions. Both versions include clustering reagents for the cBot 2 or cBot System and indexing and paired-end reagents for the HiSeq 1000 System.
• The SBS kit is available in two sizes: 200-cycle and 50-cycle. Both sizes include sequencing reagents to sequence one flow cellon the HiSeq 1000 System.
• Both kits are single-use.

For other system requirements, dual indexing considerations, and compatible products, see HiSeq 1000 Requirements & Compatibility.

Contents and Storage

When you receive your kits, promptly store kit components at the indicated temperatures to ensure proper performance.

TruSeq Cluster Kit v3

TruSeq Cluster Kit v3 reagents are shipped in four boxes on dry ice.  A flow cell, manifold, and accessories are also included.

PE Cluster Kit Box 1, Store at -25°C to -15°C

* Instead of HP7, Nextera libraries require HP11 provided in the TruSeq Dual Index Sequencing Primer Box.

PE Cluster Kit Box 2, Store at -25°C to -15°C

SR Cluster Kit Box, Store at -25°C to -15°C

Multiplex Sequencing Primer Box, Store at -25°C to -15°C

* Instead of HP8, Nextera libraries require HP12 provided in the TruSeq Dual Index Sequencing Primer Box.

Accessory Kit

TruSeq SBS Kit v3

TruSeq SBS Kit v3 reagents are shipped in two boxes on dry ice.

Reagents are labeled with a three-letter acronym plus a numeric designator that indicates the kit size. For example, if you are using CMR-50, the CMR was provided in a 50-cycle kit.

Box 1

Box 2, Store at -25°C to -15°C

TruSeq Dual Index Sequencing Primer Box, Single-Read, Store at -25° to -15°C

TruSeq Dual Index Sequencing Primer Box, Paired-End, Store at -25° to -15°C