NextSeq 500 Documentation

System Documents

SIZE

DATE

NextSeq 500 System Guide

System overview and instructions for operating and maintaining the NextSeq 500.

View Options

NextSeq 500 System Guide (15046563 v07)

8 MB

Oct 21, 2021

NextSeq 500 System Guide in Brazilian Portuguese

1 MB

Feb 22, 2022

NextSeq 500 System Guide in French

1 MB

Feb 22, 2022

NextSeq 500 System Guide in German

1 MB

Feb 22, 2022

NextSeq 500 System Guide in Italian

1 MB

Feb 22, 2022

NextSeq 500 System Guide in Japanese

4 MB

Jan 16, 2019

NextSeq 500 System Guide in Korean

6 MB

Jun 15, 2016

NextSeq 500 System Guide in Portuguese

1 MB

May 23, 2022

NextSeq 500 System Guide in Russian

1 MB

Feb 22, 2022

NextSeq 500 System Guide in Simplified Chinese

2 MB

Feb 22, 2022

NextSeq 500 System Guide in Spanish

1 MB

Feb 22, 2022

NextSeq System Custom Primers Guide

Instructions for sequencing with custom primers on the NextSeq 500 and NextSeq 550 Systems.

Download

< 1 MB

Mar 8, 2020

NextSeq System Site Prep Guide

Lab specifications and requirements to prepare a site for the the NextSeq 500 and NextSeq 550 Systems.

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NextSeq 500 and NextSeq 550 System Site Prep Guide (15045113 v06)

< 1 MB

Jul 13, 2023

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in Brazilian Portuguese

< 1 MB

May 28, 2020

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in Dutch

< 1 MB

May 28, 2020

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in French

< 1 MB

May 28, 2020

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in German

< 1 MB

May 28, 2020

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in Italian

< 1 MB

May 28, 2020

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in Japanese

1 MB

Aug 29, 2023

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in Korean

< 1 MB

Jul 21, 2023

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in Luxembourgish

< 1 MB

May 28, 2020

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in Russian

< 1 MB

May 28, 2020

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in Simplified Chinese

< 1 MB

May 28, 2020

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in Spanish

< 1 MB

May 28, 2020

NextSeq System Safety and Compliance Guides

Safety considerations, compliance markings, and regulatory statements for the NextSeq 500 and NextSeq 550 systems.

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NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide (15046564)

< 1 MB

Mar 8, 2020

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in Brazilian Portuguese

< 1 MB

Jan 7, 2021

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in French

< 1 MB

Jan 7, 2021

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in German

< 1 MB

Jan 7, 2021

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in Italian

< 1 MB

Jan 7, 2021

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in Japanese

< 1 MB

Jun 8, 2016

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in Korean

< 1 MB

Jul 14, 2021

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in Luxembourgish

< 1 MB

Jan 7, 2021

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in Russian

< 1 MB

Feb 5, 2021

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in Simplified Chinese

< 1 MB

Jan 7, 2021

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in Spanish

< 1 MB

Jan 7, 2021

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in Ukrainian

< 1 MB

Jan 7, 2021

RFID Reader Compliance Guide (15041950 v01)

2 MB

Nov 2, 2015

RFID Reader Compliance Guide in Brazilian Portuguese

2 MB

Nov 2, 2015

RFID Reader Compliance Guide in Dutch

< 1 MB

Nov 22, 2019

RFID Reader Compliance Guide in French

2 MB

Nov 2, 2015

RFID Reader Compliance Guide in German

2 MB

Nov 2, 2015

RFID Reader Compliance Guide in Italian

2 MB

Nov 2, 2015

RFID Reader Compliance Guide in Korean

2 MB

Nov 2, 2015

RFID Reader Compliance Guide in Luxembourgish

< 1 MB

Nov 22, 2019

RFID Reader Compliance Guide in Russian

2 MB

Nov 2, 2015

RFID Reader Compliance Guide in Simplified Chinese

2 MB

Nov 2, 2015

RFID Reader Compliance Guide in Spanish

2 MB

Nov 2, 2015

NextSeq System Denature and Dilute Libraries Guide – Translations

Instructions for preparing PhiX and denaturing and diluting libraries for sequencing on the NextSeq 500 and 550 Systems.

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NextSeq 500 and NextSeq 550 Sequencing Systems Denature and Dilute Libraries Guide in Japanese

2 MB

Jan 13, 2022

NextSeq 500 and 550 Systems Denature and Dilute Libraries Guide in Korean

2 MB

Nov 15, 2021

Supporting Documents

SIZE

DATE

Cluster Optimization Overview

Strategies for optimizing cluster density and preventing and diagnosing clustering issues on Illumina flow cells.
View Online Help

Apr 26, 2021

Indexed Sequencing Overview

The sequencing process for single- and dual-indexed runs on Illumina instruments.

View Online Help

Aug 13, 2024

Security and Networking

Guidelines for managing the security of the control computer on your sequencing system. Use these guidelines to configure your system and ensure a more secure operating environment.

It is strongly recommended that you review this documentation to keep your instrument secure.

View Online Help

Jan 12, 2024

Release Notes

SIZE

DATE

NextSeq System Suite Release Notes

Release notes for the NextSeq System Suite software.

View Options

NextSeq 550 RUO v4.2.0 Software Customer Release Notes

< 1 MB

May 31, 2023

NextSeq v4.0.2 Software Release Notes (1000000132193 v00)

< 1 MB

Oct 1, 2020

NextSeq v4.0.1 Software Release Notes (1000000101366 v01)

< 1 MB

Sep 2, 2020

NextSeq v4.0.1 Software Release Notes Translated into Simplified Chinese

< 1 MB

Nov 4, 2019

NextSeq v4.0 Software Release Notes (1000000070338 v03)

< 1 MB

Sep 2, 2020

NextSeq RUO Software Suite Installer Release Notes (1000000051580 v00)

< 1 MB

Mar 13, 2018

NextSeq v2.2.0 Software Release Notes (1000000056025 v00)

< 1 MB

May 14, 2018

NextSeq v2.1.3 Software Release Notes (1000000017010 v01)

< 1 MB

Oct 24, 2017

NextSeq Software Release Notes, v2.0.2 (1000000004907-00)

< 1 MB

Nov 10, 2015

NextSeq System Software v2.0.1 Release Notes (15051678 K)

< 1 MB

Jun 24, 2015

NextSeq System Software v1.3.2 Release Notes (15071669 A)

< 1 MB

May 27, 2015

Product Literature	TYPE	SIZE	DATE
Laboratory Experience Using Illumina Next-Generation Sequencing for Noninvasive Prenatal Testing Application Note Download	Application note	< 1 MB	Mar 27, 2015

NextSeq 500 Documentation

NextSeq 500 System

Documentation

System Documents

SIZE

DATE

NextSeq 500 System Guide

NextSeq System Custom Primers Guide

NextSeq System Site Prep Guide

NextSeq System Safety and Compliance Guides

NextSeq System Denature and Dilute Libraries Guide – Translations

Supporting Documents

SIZE

DATE

Cluster Optimization Overview

Indexed Sequencing Overview

Security and Networking

Release Notes

SIZE

DATE

NextSeq System Suite Release Notes

Product Literature

TYPE

SIZE

DATE

Laboratory Experience Using Illumina Next-Generation Sequencing for Noninvasive Prenatal Testing Application Note

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NextSeq 500 Documentation

NextSeq 500 System

Documentation

System Documents

SIZE

DATE

NextSeq 500 System Guide

NextSeq System Custom Primers Guide

NextSeq System Site Prep Guide

NextSeq System Safety and Compliance Guides

NextSeq System Denature and Dilute Libraries Guide – Translations

Supporting Documents

SIZE

DATE

Cluster Optimization Overview

Indexed Sequencing Overview

Security and Networking