NextSeq 500 Compatible Products

Sample Information

Choose from the following options to record information about your samples, such as sample ID and which index adapters are used for pooling.

Library Preparation

See NextSeq Applications and Methods for possible applications.

After library prep, denature and dilute libraries to a loading concentration of ~1.8 pM. For instructions, see NextSeq System Denature and Dilute Libraries Guide.

Run Components

Use the compatible version of the NextSeq Control Software (NCS) for your kit. For kit details, see NextSeq 500 Reagent Kit.

Kit Version

NCS Version

NextSeq 500/550 v2.5 Kit

NCS v2.2.0

NextSeq 500/550 v2 Kit

NCS v1.4, or later

NextSeq 500/550 Kit (v1)

NCS v1.3, or earlier

TG NextSeq 500 High Output Kit

NCS v1.3

Data Analysis

Use Sequencing Analysis Viewer or BaseSpace Sequence Hub to monitor real-time quality metrics during a run.

To analyze sequencing data, choose from the following options.