NovaSeq 6000 Training

Recorded Webinar (April 2019) | Bcl2Fastq is a Linux-based software that converts base call files generated from an Illumina sequencing run to FASTQ files as well as demultiplex samples. This webinar focuses on Bcl2fastq v2+ and is targeted for new to intermediate users who are interested in the configurable options that this command line program allows. We will cover the following topics: requirements to run Bcl2Fastq, command line basics, commonly used options, frequently asked ‘How To….’ questions.

In this video, an Illumina Field Applications Scientist discusses what to expect during the pre-installation, shipping, and delivery stages. We’ll highlight important steps in the process and introduce you to available resources. Note that, depending on your region, the steps shown in this video may not apply to the iSeq 100 system.

Recorded Webinar (February 2021) | Join Illumina Technical Support for a discussion on the NovaSeq 6000 platform. This webinar is targeted towards users with a basic understanding of the Illumina sequencing workflow. We will cover the following topics: features of NovaSeq 6000, end-to-end workflow, library preparation considerations, guidance on run analysis, and tips and best practices for running and maintaining your instrument.

This course introduces lab requirements, computing requirements, and delivery considerations for the NovaSeq 6000 System. It also discusses the consumables required for running and maintaining the instrument.

Recorded Webinar (December 2020) | The Sequencing Analysis Viewer (SAV) Software is an application where users can view important quality metrics generated during sequencing runs. This webinar will provide a guided tour for beginners on how to use SAV, as well as tips and tricks for reviewing the most useful information for sequencing runs. This webinar is targeted at new users and will go over the following topics: how to load data into SAV, what metrics are available in each tab of the software, and understanding which are the most valuable metrics for run review and where to find them.

Recorded Webinar (January 2021) | Illumina next-generation sequencing technology allows for massive parallel sequencing. Illumina Technical Support invites you to join us for a presentation and discussion on sample preparation, cluster generation and sequencing by synthesis chemistry. This webinar is targeted to those new to next-generation sequencing and will discuss the following topics: Illumina library construction, cluster generation methods by platform, sequencing by synthesis, and multiplexing and primary analysis.

Learn about the benefits of instrument performance monitoring through Illumina Proactive, a service in which instrument performance data are sent to Illumina to enable proactive maintenance.

This video shares IT requirements for implementing Illumina Proactive, an instrument performance monitoring service provided by Illumina that can increase instrument uptime, improve operational efficiency, and reduce the risk of lost resources.

This video demonstrates the NovaSeq™ Xp workflow, including NovaSeq flow cell preparation, ExAmp and library master mix preparation, and manually loading libraries onto the flow cell.

Although over clustering is not possible on patterned flow cell, loading a library with a suboptimal concentration negatively impacts run data. This video discusses preventing and diagnosing common clustering issues on patterned flow cells.

This video discusses optimal cluster density and how over and under-clustering can affect your sequencing data. It also examines common clustering issues and ways to prevent them.