TruSeq Stranded mRNA Library Prep (48 Samples)
Box 1
| Quantity |
Reagent |
Description |
Storage |
|---|
| 1 |
RPB |
RNA Purification Beads |
2°C to 8°C |
| 1 |
DTE |
CTE Dilution Tube |
Room temperature |
| 1 |
DTA |
CTA Dilution Tube |
Room temperature |
| 1 |
DTL |
CTL Dilution Tube |
Room temperature |
Box2
| Quantity |
Reagent |
Description |
Storage |
|---|
| 1 |
BBB |
Bead Binding Buffer |
2°C to 8°C |
| 1 |
BWB |
Bead Washing Buffer |
2°C to 8°C |
| 1 |
ELB |
Elution Buffer |
2°C to 8°C |
| 1 |
FPF |
Fragment, Prime, Finish Mix |
-25°C to -15°C |
Core Library Prep Box 1, Store at -25°C to -15°C
| Quantity |
Reagent |
Description |
|---|
| 1 |
ATL |
A-Tailing Mix |
| 1 |
CTA |
A-Tailing Control |
| 1 |
CTE |
End Repair Control |
| 1 |
CTL |
Ligation Control |
| 1 |
LIG |
Ligation Mix |
| 1 |
RSB |
Resuspension Buffer |
| 1 |
STL |
Stop Ligation Buffer |
Core Library Prep Box 2, Store at -25°C to -15°C
| Quantity |
Reagent |
Description |
|---|
| 1 |
PMM |
PCR Master Mix |
| 1 |
PPC |
PCR Primer Cocktail |
| 1 |
FSA |
First Strand Synthesis Act D Mix |
| 1 |
SMM |
Second Strand Marker Master Mix |
TruSeq Stranded mRNA Library Prep (96 Samples)
A quantity of two of each box is included for the 96 sample workflow.
Box 1
| Quantity |
Reagent |
Description |
Storage |
|---|
| 2 |
RPB |
RNA Purification Beads |
2°C to 8°C |
| 1 |
DTE |
CTE Dilution Tube |
Room temperature |
| 1 |
DTA |
CTA Dilution Tube |
Room temperature |
| 1 |
DTL |
CTL Dilution Tube |
Room temperature |
Box2
| Quantity |
Reagent |
Description |
Storage |
|---|
| 2 |
BBB |
Bead Binding Buffer |
2°C to 8°C |
| 2 |
BWB |
Bead Washing Buffer |
2°C to 8°C |
| 2 |
ELB |
Elution Buffer |
2°C to 8°C |
| 2 |
FPF |
Fragment, Prime, Finish Mix |
-25°C to -15°C |
Core Library Prep Box 1, Store at -25°C to -15°C
| Quantity |
Reagent |
Description |
|---|
| 1 |
ATL |
A-Tailing Mix |
| 1 |
CTA |
A-Tailing Control |
| 1 |
CTE |
End Repair Control |
| 1 |
CTL |
Ligation Control |
| 1 |
LIG |
Ligation Mix |
| 1 |
RSB |
Resuspension Buffer |
| 1 |
STL |
Stop Ligation Buffer |
Core Library Prep Box 2, Store at -25°C to -15°C
| Quantity |
Reagent |
Description |
|---|
| 2 |
PMM |
PCR Master Mix |
| 2 |
PPC |
PCR Primer Cocktail |
| 2 |
FSA |
First Strand Synthesis Act D Mix |
| 2 |
SMM |
Second Strand Marker Master Mix |