TruSight Tumor 170 Kit Support

TruSight Tumor 170 Kit Contents & Storage

Library Prep

Box 1, RNA (Pre-Amp)
Quantity Reagent Description Storage
1 FSM First Strand Synthesis Mix -25°C to -15°C
1 SSM Second Strand Mix -25°C to -15°C
1 EPH3 Elute, Prime Fragment High Mix 3 -25°C to -15°C
1 RVT Reverse Transcriptase -25°C to -15°C
Box 2, DNA (Pre-Amp)
Quantity Reagent Description Storage
2 ERA1-A End Repair A-tailing Enzyme Mix 1 -25°C to -15°C
2 ERA1-B End Repair A-tailing Buffer 1 -25°C to -15°C
2 ALB1 Adapter Ligation Buffer 1 -25°C to -15°C
2 LIG3 DNA Ligase 3 -25°C to -15°C
2 SUA1 Short Universal Adapters 1 -25°C to -15°C
2 STL Stop Ligation Buffer -25°C to -15°C
2 EPM Enhanced PCR Mix -25°C to -15°C
Box 3 (Pre-Amp)
Quantity Reagent Description Storage
1 RSB Resuspension Buffer 2°C to 8°C or -25°C to -15°C
2 SPB Sample Purification 2°C to 8°C
1 TEB TE Buffer 2°C to 8°C
Box 4, Unique PCR Index Primer Mixes (Pre-Amp)
Quantity Reagent Description Storage
1 UP01 Unique Index Mix Primer 01 -25°C to -15°C
1 UP02 Unique Index Mix Primer 02 -25°C to -15°C
1 UP03 Unique Index Mix Primer 03 -25°C to -15°C
1 UP04 Unique Index Mix Primer 04 -25°C to -15°C
1 UP05 Unique Index Mix Primer 05 -25°C to -15°C
1 UP06 Unique Index Mix Primer 06 -25°C to -15°C
1 UP07 Unique Index Mix Primer 07 -25°C to -15°C
1 UP08 Unique Index Mix Primer 08 -25°C to -15°C
1 UP09 Unique Index Mix Primer 09 -25°C to -15°C
1 UP10 Unique Index Mix Primer 10 -25°C to -15°C
1 UP11 Unique Index Mix Primer 11 -25°C to -15°C
1 UP12 Unique Index Mix Primer 12 -25°C to -15°C
1 UP13 Unique Index Mix Primer 13 -25°C to -15°C
1 UP14 Unique Index Mix Primer 14 -25°C to -15°C
1 UP15 Unique Index Mix Primer 15 -25°C to -15°C
1 UP16 Unique Index Mix Primer 16 -25°C to -15°C
Box 5, Combinatorial PCR Index Primer Mixes (Pre-Amp)
Quantity Reagent Description Storage
1 CP01 Combinatorial Index Primer Mix 01 -25°C to -15°C
1 CP02 Combinatorial Index Primer Mix 02 -25°C to -15°C
1 CP03 Combinatorial Index Primer Mix 03 -25°C to -15°C
1 CP04 Combinatorial Index Primer Mix 04 -25°C to -15°C
1 CP05 Combinatorial Index Primer Mix 05 -25°C to -15°C
1 CP06 Combinatorial Index Primer Mix 06 -25°C to -15°C
1 CP07 Combinatorial Index Primer Mix 07 -25°C to -15°C
1 CP08 Combinatorial Index Primer Mix 08 -25°C to -15°C
1 CP09 Combinatorial Index Primer Mix 09 -25°C to -15°C
1 CP10 Combinatorial Index Primer Mix 10 -25°C to -15°C
1 CP11 Combinatorial Index Primer Mix 11 -25°C to -15°C
1 CP12 Combinatorial Index Primer Mix 12 -25°C to -15°C
1 CP13 Combinatorial Index Primer Mix 13 -25°C to -15°C
1 CP14 Combinatorial Index Primer Mix 14 -25°C to -15°C
1 CP15 Combinatorial Index Primer Mix 15 -25°C to -15°C
1 CP16 Combinatorial Index Primer Mix 16 -25°C to -15°C

Enrichment

Box 6 (Post-Amp)
Quantity Reagent Description Storage
2 TCB1 Target Capture Buffer 1 2°C to 8°C
2 SMB Streptavidin Magnetic Beads 2°C to 8°C
2 HP3 2N NaOH 2°C to 8°C
2 ET2 Elution Target 2 2°C to 8°C
2 LNB1 Library Normalization Beads 1 2°C to 8°C
2 LNW1 Library Normalization Wash 1 2°C to 8°C
1 RSB Resuspension Buffer 2°C to 8°C or -25°C to -15°C
2 SPB Sample Purification Beads 2°C to 8°C
2 LNS2 Library Normalization Storage 2 15°C to 30°C

Remove the LNS2 tubes from the 2°C to 8°C box and store at 15°C to 30°C.

Box 7 (Post-Amp))
Quantity Reagent Description Storage
2 TCA1 Target Capture Additive 1 -25°C to -15°C
4 EEW2 Enhanced Enrichment Wash 2 -25°C to -15°C
2 EE2 Enrichment Elution 2 -25°C to -15°C
2 EPM Enhanced PCR Mix -25°C to -15°C
2 PPC3 PCR Primer Cocktail 3 -25°C to -15°C
2 LNA1 Library Normalization Additives 1 -25°C to -15°C
Box 8, TruSight Tumor 170 Content Set (DNA Only)
Quantity Reagent Description Storage
1 OPD1 Oncology DNA Probes Master Pool -25°C to -15°C
Box 9, TruSight Tumor 170 Content Set (RNA Only)
Quantity Reagent Description Storage
1 OPR1 Oncology RNA Probes Master Pool -25°C to -15°C
Contact Us
Technical Support
  • techsupport@illumina.com

View All Contacts

Share With Tech Support

Get instructions for sharing your desktop while working with Technical Support.

Share Desktop
Other Support
For Research Use Only

Not for use in diagnostic procedures (except as specifically noted).

Innovative technologies

At Illumina, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. It is mission critical for us to deliver innovative, flexible, and scalable solutions to meet the needs of our customers. As a global company that places high value on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics.

All trademarks are the property of Illumina, Inc. or their respective owners.
For specific trademark information, see www.illumina.com/company/legal.html.

© 2024 Illumina, Inc. All rights reserved.