cBot Compatible Products

Reagent Kits

The cBot System uses the following reagent kits to cluster or rehybridize HiSeq flow cells. When clustering is complete, the flow cell is sequenced on a HiSeq system using reagents from both the cluster and SBS kits and any other supplementary kits.

For catalog IDs, contents, storage, and compatible HiSeq Control Software (HCS), see the support pages for the compatible HiSeq system.

• HiSeq X System
• HiSeq 4000 or HiSeq 3000 System
• HiSeq 2500 or HiSeq 1500 System
• HiSeq 2000 or HiSeq 1000 System

Cluster Kits for HiSeq X Flow Cells

• HiSeq X Ten Reagent Kit v2.5
• HiSeq X Ten Reagent Kit v2.5 (10 pack)
• HiSeq X Five Reagent Kit v2.5
• HiSeq X Five Reagent Kit v2.5 (10 pack)

Cluster Kits for HiSeq 3000/4000 Flow Cells

• HiSeq 3000/4000 SR Cluster Kit
• HiSeq 3000/4000 PE Cluster Kit

Cluster Kits for HiSeq v4 and TruSeq v3 Flow Cells

HiSeq v4 and TruSeq v3 flow cells are high-output flow cells compatible with the HiSeq 2500, HiSeq 1500, HiSeq 2000, and HiSeq 1000 Systems.

• HiSeq SR Cluster Kit v4
• HiSeq PE Cluster Kit v4
• TruSeq SR Cluster Kit v3
• TruSeq PE Cluster Kit v3

Cluster Kits for HiSeq Rapid v2 Flow Cells

Cluster the HiSeq Rapid v2 flow cell on the cBot only when using the HiSeq Rapid Duo cBot Sample Loading Kit to load a separate library in each lane. Otherwise, both clustering and sequencing are performed on the HiSeq 2500 or HiSeq 1500.

Read 1 Sequencing Primer for Nextera Libraries

Sequencing Nextera libraries on a TruSeq v3 flow cell requires HP10 from one of the following kits:

• TruSeq Dual Index Sequencing Primer Box, Single-Read
• TruSeq Dual Index Sequencing Primer Box, Paired-End

Rehybridization Kits

The rehybridization kits repeat Read 1 primer hybridization to save a flow cell. For more information on these kits, see the rehybridization guide.

• HiSeq X cBot Multi-Primer Rehybridization Kit v2 (catalog # GD-305-2001)
• HiSeq 3000/4000 cBot Multi-Primer Rehybridization Kit (catalog # GD-310-1001)
• TruSeq v2 cBot Multi-Primer Rehybridization Kit (catalog # GD-304-2001)

Run Components

Use the compatible version of cBot software for your kit.