This product was formerly named IDT for Illumina Nextera DNA UD Indexes.
IDT for Illumina DNA/RNA UD Indexes are extension-based unique dual (UD) indexes with unrelated adapters for both index reads. The index adapter sequences are 10 bases long.
Before preparing libraries, create a sample sheet to record the index adapter combinations and other information about your samples. Sample sheet options and requirements depend on the instrument. For compatibility information, see the support pages for your instrument.
When using Local Run Manager or Illumina Experiment Manager (IEM) to prepare a sample sheet, consider the following requirements for IDT for Illumina DNA/RNA UD Indexes:
IDT for Illumina DNA/RNA UD Indexes are compatible with the following library prep kits.
The following instruments can sequence libraries appended with IDT for Illumina DNA/RNA UD Indexes. For library prep kit and instrument compatibility, see the support pages for your kit or instrument.
Enter 10 cycles each for Index 1 and Index 2 to ensure that the software reads the complete 10-base index adapter sequence. For the recommended number of Read 1 and Read 2 cycles, see the Compatible Products page for your kit.
The system guide for each instrument provides instructions for entering cycles for Read 1, Index 1, Index 2, and Read 2.