Before preparing libraries, record information about your samples using the available options for your sequencing system.
After library preparation, denature and dilute libraries to the appropriate loading concentration using instructions for standard-normalized libraries.
The following table shows the recommended loading concentration, number of libraries per flow cell, and reads per sample for the MiSeq and NextSeq Systems.
System |
Loading Concentration (pM) | Number of Libraries per Flow Cell |
Reads per Sample (M) |
| MiniSeq | 2–2.6 | 8 | 3 |
MiSeq (v3 reagents) |
20–30 |
8 |
3 |
NextSeq |
2–3 | 24 | 5.4 |